CTF2P Mouse
Escherichia Coli.
Cardiotrophin-2, CT-2, Neuropoietin, Np, Ctf2, Gm494.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Neuropoietin Mouse Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 182 amino acids and having a molecular mass of approximately 19.7kDa.
CTF2P is purified by proprietary chromatographic techniques.
Product Specs
CTF2P, also known as Neuropoietin, belongs to the IL-6 family of cytokines. It is the result of a gene duplication involving cardiotrophin-1 (CT-1) and is part of a subfamily within the IL-6 family that includes CT-1, CLC, and CTNF. CTF2P contributes to an increase in platelet count, often associated with splenomegaly, and plays a role in the development and maturation of neuronal precursors.
Recombinant Mouse Neuropoietin, produced in E. coli, is a polypeptide chain that is not glycosylated. It consists of 182 amino acids, resulting in a molecular weight of approximately 19.7 kDa. The purification of CTF2P is achieved through specialized chromatographic methods.
Sterile, white powder that has been lyophilized (freeze-dried) and filtered.
The protein is lyophilized from a concentrated solution (0.2 μm filtered) containing 20mM Tris-HCl at pH 8.0, 0.5mM DTT, and 500mM NaCl.
For reconstitution of the lyophilized Neuropoietin, it is recommended to use sterile PBS at a concentration of at least 100 μg/ml. This solution can be further diluted with other aqueous solutions as needed.
Lyophilized CTF2P remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, Neuropoietin should be stored at 4°C for a period of 2-7 days. For long-term storage, it is advisable to store it at -18°C. Avoid repeated freeze-thaw cycles.
The purity of the product is determined using the following methods and is guaranteed to be greater than 98.0%:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
The ED50, determined by a cell proliferation assay using human TF-1 cells, is less than 200 ng/ml. This corresponds to a specific activity greater than 5000 IU/mg.
Cardiotrophin-2, CT-2, Neuropoietin, Np, Ctf2, Gm494.
Escherichia Coli.
APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA.
Product Science Overview
Mouse neuropoietin is synthesized as a 204 amino acid precursor that contains a 22 amino acid signal sequence and a 192 amino acid mature segment . The secreted molecule is characterized by the presence of four alpha-helices, typical of hematopoietic superfamily molecules . The recombinant form of mouse neuropoietin is typically produced in E. coli and is available in both carrier-free and carrier protein formulations .
Recombinant mouse neuropoietin is used in various research applications, including cell culture and ELISA standards. It is typically lyophilized from a filtered solution and can be reconstituted in sterile PBS or aqueous buffer containing 0.1% BSA . For optimal stability, it should be stored at -20 to -70°C and should avoid repeated freeze-thaw cycles .
