NEIL2 Human
Description
NEIL2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
DWRDPSPRLV LHFGGGGFLA FYNCQLSWSS SPVVTPTCDI LSEKFHRGQA LEALGQAQPV CYTLLDQRYF SGLGNIIKNE ALYRAGIHPL SLGSVLSASR REVLVDHVVE FSTAWLQGKF QGRPQHTQVY QKEQCPAGHQ VMKEAFGPED GLQRLTWWCP QCQPQLSEEP EQCQFS.
Product Science Overview
NEIL2 is a single, non-glycosylated polypeptide chain consisting of 356 amino acids, with a molecular mass of approximately 39.4 kDa . It is produced in E. coli and is fused to a 24 amino acid His-tag at the N-terminus, which facilitates its purification through proprietary chromatographic techniques .
The primary function of NEIL2 is to recognize and excise oxidatively damaged bases from DNA. It does this by cleaving the glycosidic bond between the damaged base and the sugar-phosphate backbone, creating an apurinic/apyrimidinic (AP) site. This AP site is then further processed by other enzymes in the BER pathway to restore the DNA to its undamaged state .
The recombinant NEIL2 protein is expressed in E. coli and purified to a high degree of purity, greater than 85% as determined by SDS-PAGE . The protein is formulated in a sterile, filtered colorless solution containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, and 0.1M NaCl . This formulation ensures the stability and activity of the protein during storage and experimental use.
NEIL2 is widely used in research to study DNA repair mechanisms, particularly those involving oxidative damage. It is also used in various biochemical assays to understand the role of DNA glycosylases in maintaining genomic integrity. Researchers utilize NEIL2 to investigate the molecular mechanisms underlying various diseases, including cancer, where oxidative DNA damage plays a significant role.
For optimal stability, NEIL2 should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s activity.
