Introduction
NCS1, a member of the neuronal calcium sensor gene family, encodes a calcium-binding protein primarily found in neurons. This protein plays a crucial role in regulating G protein-coupled receptor phosphorylation in a calcium-dependent manner, demonstrating its ability to function similarly to calmodulin. NCS1 is associated with secretory granules and influences both synaptic transmission and plasticity. Notably, it regulates GRK1 and can substitute for calmodulin in certain processes. Furthermore, NCS1 stimulates PI4KB kinase activity and participates in long-term synaptic plasticity through its interaction with PICK1. Additionally, NCS1 contributes to neuron differentiation by inhibiting the activity of N-type voltage-gated calcium channels.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is provided at a concentration of 1mg/ml and is formulated in a solution containing PBS at a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage of up to one month, the antibody should be kept at 4°C. For longer storage durations, it is recommended to store the antibody at -20°C. To maintain product integrity, it is crucial to avoid repeated cycles of freezing and thawing.
Stability / Shelf Life
The antibody remains stable for a period of 12 months when stored at -20°C. At a temperature of 4°C, the antibody retains its stability for up to 1 month.
Applications
This antibody has undergone rigorous testing using ELISA, Western blot analysis, and Flow cytometry to confirm its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application. Therefore, users are advised to perform their own titrations to determine the optimal working dilution for their particular experimental conditions.
Synonyms
Frequenin homolog, FLUP, FREQ, Neuronal calcium sensor 1,
Frequenin-like protein, Frequenin-like ubiquitous protein, NCS-1, NCS1,
DKFZp761L1223.
Purification Method
NCS1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human NCS1 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human NCS1 protein 1-190 amino acids purified
from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
