NCS1 213 a.a. Human

Neuronal Calcium Sensor 1 213 a.a. Human Recombinant
Cat. No.
BT17488
Source
Escherichia Coli.
Synonyms
Neuronal Calcium Sensor 1, Frequenin-Like Ubiquitous Protein, Frequenin-Like Protein, Frequenin Homolog, FREQ, FLUP, Frequenin (Drosophila) Homolog, Frequenin Homolog (Drosophila), FREQ, NCS-1, Neuronal calcium sensor 1.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NCS1 213 a.a. Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a) and having a molecular mass of 24.3kDa.
NCS1 213 a.a. is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NCS1, a member of the neuronal calcium sensor family, is primarily found in neurons and binds calcium. It plays a role in regulating G protein-coupled receptor phosphorylation in a calcium-dependent manner and can function similarly to calmodulin. NCS1 is associated with secretory granules and influences synaptic transmission and plasticity. It regulates GRK1 and acts as a substitute for calmodulin. Additionally, NCS1 stimulates PI4KB kinase activity and contributes to long-term synaptic plasticity by interacting with PICK1. Furthermore, it participates in neuron differentiation by inhibiting the activity of N-type voltage-gated calcium channels.
Description
This product consists of a single, non-glycosylated polypeptide chain comprising 213 amino acids (specifically, amino acids 1 to 190). It has a molecular weight of 24.3 kDa and is produced through recombinant expression in E. coli. The NCS1 protein sequence is fused at the N-terminus with a 23 amino acid His-tag and is purified using proprietary chromatographic methods.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The NCS1 protein is supplied in a solution with a concentration of 1 mg/ml. The solution contains 20mM Tris-HCl buffer at pH 8.0, 0.15M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. It's important to avoid repeatedly freezing and thawing the product.
Purity
The purity of this product, as determined by SDS-PAGE analysis, is greater than 95%.
Synonyms
Neuronal Calcium Sensor 1, Frequenin-Like Ubiquitous Protein, Frequenin-Like Protein, Frequenin Homolog, FREQ, FLUP, Frequenin (Drosophila) Homolog, Frequenin Homolog (Drosophila), FREQ, NCS-1, Neuronal calcium sensor 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGKSNSK LKPEVVEELT RKTYFTEKEV QQWYKGFIKD CPSGQLDAAG FQKIYKQFFP FGDPTKFATF VFNVFDENKD GRIEFSEFIQ ALSVTSRGTL DEKLRWAFKL YDLDNDGYIT RNEMLDIVDA IYQMVGNTVE LPEEENTPEK RVDRIFAMMD KNADGKLTLQ EFQEGSKADP SIVQALSLYD GLV.

Product Science Overview

Introduction

Neuronal Calcium Sensor 1 (NCS1) is a member of the neuronal calcium sensor family of proteins, which play a crucial role in regulating various cellular processes by sensing changes in intracellular calcium levels. The human recombinant form of NCS1, consisting of 213 amino acids, is a valuable tool for research and therapeutic applications.

Structure and Function

NCS1 is a small, soluble protein that binds calcium ions with high affinity. It contains four EF-hand motifs, which are helix-loop-helix structures that coordinate calcium binding. Upon binding calcium, NCS1 undergoes a conformational change that allows it to interact with target proteins and modulate their activity.

Biological Role

NCS1 is predominantly expressed in the brain, where it is involved in various neuronal functions, including:

  • Synaptic plasticity: NCS1 modulates neurotransmitter release and synaptic strength, contributing to learning and memory processes.
  • Signal transduction: By interacting with various signaling molecules, NCS1 plays a role in intracellular signaling pathways that regulate neuronal survival, differentiation, and growth.
  • Regulation of ion channels: NCS1 influences the activity of ion channels, such as voltage-gated calcium channels, thereby affecting neuronal excitability and communication.
Recombinant Production

The human recombinant form of NCS1 (213 a.a.) is produced using recombinant DNA technology. This involves cloning the NCS1 gene into an expression vector, which is then introduced into a host organism (typically bacteria or yeast) for protein production. The recombinant protein is subsequently purified using chromatographic techniques to obtain a high-purity product suitable for research and therapeutic use.

Applications

Recombinant NCS1 is used in various research applications, including:

  • Neuroscience research: Studying the role of NCS1 in neuronal function and its involvement in neurological disorders.
  • Drug discovery: Screening for compounds that modulate NCS1 activity, which could lead to the development of new therapeutic agents for neurological diseases.
  • Biochemical studies: Investigating the structure-function relationship of NCS1 and its interactions with other proteins.

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THE BIOTEK
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