NCR3LG1 Human

Natural Cytotoxicity Triggering Receptor 3 Ligand 1 Human Recombinant
Cat. No.
BT28481
Source

HEK293 Cells

Synonyms

B7H6, B7-H6, B7 Homolog 6

Appearance
Sterile Filtered clear solution.
Purity

Greater than 95% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NCR3LG1 Human Recombinant produced in HEK is a single, glycosylated polypeptide chain containing 477 amino acids (25-262 a.a) and having a molecular mass of 53.6 kDa. NCR3LG1 is fused to a 239 amino acid hIgG-His tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The B7 proteins play a critical role in regulating the adaptive immune system. NCR3LG1, a member of the B7 family, is found on the surface of tumor cells. When NCR3LG1 interacts with the NKp30 protein, it triggers cytotoxicity and activates natural killer cells, which are essential components of the immune system. While humans and rats possess a single NCR3LG1 gene, the Xenopus genome contains multiple B7H6 genes clustered together. NCR3LG1 acts as a ligand for the NK cell activating receptor NKp30, making it a potential target for CAR T-cell therapy aimed at treating various tumor types. Notably, NCR3LG1 is present on numerous primary human tumors, including leukemia, lymphoma, and gastrointestinal stromal tumors, while its expression on healthy tissues is not constitutive.
Description
Recombinant Human NCR3LG1, produced in HEK cells, is a single polypeptide chain that is glycosylated. It comprises 477 amino acids (specifically, amino acids 25-262) and exhibits a molecular weight of 53.6 kDa. The NCR3LG1 protein is fused to a 239 amino acid hIgG-His tag at its C-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The NCR3LG1 protein solution has a concentration of 0.5 mg/ml and is prepared in a buffer consisting of 1X PBS (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is determined to be greater than 95% using SDS-PAGE analysis.
Biological Activity
Biological activity is assessed using a functional ELISA with Human NCR3. The ED50 range is found to be ≤500 ng/ml.
Synonyms

B7H6, B7-H6, B7 Homolog 6

Source

HEK293 Cells

Amino Acid Sequence

DLKVEMMAGG TQITPLNDNV TIFCNIFYSQ PLNITSMGIT WFWKSLTFDK EVKVFEFFGD HQEAFRPGAI VSPWRLKSGD ASLRLPGIQL EEAGEYRCEV VVTPLKAQGT VQLEVVASPA SRLLLDQVGM KENEDKYMCE SSGFYPEAIN ITWEKQTQKF PHPIEISEDV ITGPTIKNMD GTFNVTSCLK LNSSQEDPGT VYQCVVRHAS LHTPLRSNFT LTAARHSLSE TEKTDNFS

LE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH

Product Science Overview

Introduction

Natural Cytotoxicity Triggering Receptor 3 Ligand 1 (NCR3LG1), also known as B7-H6, is a protein encoded by the NCR3LG1 gene in humans. This ligand is a member of the B7 family and plays a crucial role in the immune system, particularly in the activation of natural killer (NK) cells. The recombinant form of this protein is often used in research to study its functions and interactions.

Gene and Protein Structure

The NCR3LG1 gene is located on chromosome 11 and encodes a protein that is part of the immunoglobulin superfamily. The protein structure includes an Ig-like domain, which is essential for its interaction with receptors on NK cells. The gene has several aliases, including B7-H6 and DKFZp686O24166 .

Function and Mechanism

NCR3LG1 is primarily expressed on the surface of tumor cells and plays a pivotal role in the immune response against cancer. It interacts with the NKp30 receptor (NCR3) on NK cells, triggering their activation and cytotoxic response . This interaction leads to the destruction of tumor cells, making NCR3LG1 a critical component in the body’s natural defense against cancer .

Clinical Significance

The expression of NCR3LG1 is associated with various types of cancers, including hematologic malignancies and solid tumors. Its presence on tumor cells and its ability to activate NK cells make it a potential target for cancer immunotherapy . Researchers are exploring ways to harness this interaction to develop treatments that enhance the immune system’s ability to fight cancer.

Research and Applications

Human recombinant NCR3LG1 is widely used in research to study its role in immune responses and its potential therapeutic applications. Studies have shown that manipulating the expression of NCR3LG1 or its interaction with NKp30 can influence the effectiveness of NK cell-mediated cytotoxicity . This has significant implications for developing new cancer treatments and understanding the mechanisms of immune evasion by tumors.

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