MYL6B Human

Myosin Light Chain 6B Human Recombinant
Cat. No.
BT15732
Source
E.coli.
Synonyms
Myosin light chain 6B, Myosin light chain 1 slow-twitch muscle A isoform, MLC1sa, Smooth muscle and nonmuscle myosin light chain alkali 6B, MYL6B, MLC1SA.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MYL6B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (1-208) and having a molecular mass of 25.2 kDa.
The MYL6B is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Myosin Light Chain 6B (MYL6B) is a crucial component of smooth muscle and non-muscle myosin complexes, playing a vital role in regulating the heavy chain. The contractile activity of smooth muscle is modulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain, facilitated by myosin light chain kinase. Interestingly, MYL6B does not bind to calcium during the contraction process. This protein predominantly exists as a hexamer, composed of four light chains and two heavy chains. MYL6B typically interacts with Myosin Va, an actin-based motor protein known for its large step movements. Its expression is widespread across various tissues, including neurons, with the highest expression observed in smooth muscle tissue.
Description
Recombinantly produced in E. coli, MYL6B Human Recombinant is a non-glycosylated polypeptide chain that is devoid of any glycosylation modifications. It encompasses 231 amino acids (residues 1-208) and possesses a molecular weight of 25.2 kDa. For purification purposes, a 23-amino acid His-Tag is fused to the N-terminus of the MYL6B protein, facilitating its isolation through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MYL6B solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 2 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the MYL6B solution should be stored at 4°C. For extended storage, it is recommended to store the solution in frozen aliquots at -20°C. To further enhance long-term stability during freezing, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. To maintain protein integrity, repeated cycles of freezing and thawing should be avoided.
Purity
The purity of MYL6B Human Recombinant is determined to be greater than 95% as assessed by SDS-PAGE analysis.
Synonyms
Myosin light chain 6B, Myosin light chain 1 slow-twitch muscle A isoform, MLC1sa, Smooth muscle and nonmuscle myosin light chain alkali 6B, MYL6B, MLC1SA.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPPKKDV PVKKPAGPSI SKPAAKPAAA GAPPAKTKAE PAVPQAPQKT QEPPVDLSKV VIEFNKDQLE EFKEAFELFD RVGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEL KSRRVDFETF LPMLQAVAKN RGQGTYEDYL EGFRVFDKEG NGKVMGAELR HVLTTLGEKM TEEEVETVLA GHEDSNGCIN YEAFLKHILS V.

Product Science Overview

Structure and Function

MYL6B is a regulatory light chain of myosin that does not bind calcium . It plays a crucial role in muscle contraction and cellular movement by interacting with myosin II heavy chains to form non-muscle myosin II holoenzymes (NMII) . These holoenzymes are essential for various cellular processes, including cytokinesis, cell migration, and maintenance of cell shape .

Role in Disease

MYL6B has been implicated in several diseases, including brachydactyly, type E1, a condition characterized by shortened fingers and toes . Additionally, MYL6B has been found to promote MDM2-mediated degradation of the p53 protein, which is a critical tumor suppressor . This interaction suggests that MYL6B may play a role in the development of certain cancers, such as hepatocellular carcinoma (HCC) .

Research and Applications

Research on MYL6B has revealed its potential as a target for cancer therapy. By inhibiting the ATPase activity of myosin II heavy chains, scientists have been able to block the degradation of p53, thereby suppressing the clonogenic ability and increasing the apoptosis level of HCC cell lines . This finding highlights the importance of MYL6B in cancer research and its potential as a therapeutic target.

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Source
Escherichia Coli.
THE BIOTEK
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