MYL6 Human

MYL6 is a myosin alkali light chain expressed in both smooth muscle and non-muscle tissues. It is involved in generating the force required for cellular movements, thus playing an essential role in overall cellular function . The recombinant form of MYL6 is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 171 amino acids with a molecular mass of 19.1 kDa .

The recombinant MYL6 protein is typically fused with a 20 amino acid His tag at the N-terminus to facilitate purification. The protein is purified using proprietary chromatographic techniques to achieve a purity greater than 90%, as determined by SDS-PAGE . The formulation of MYL6 includes a 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, and 10% glycerol .

Recombinant MYL6 is used extensively in laboratory research to study muscle contraction, cellular movement, and related biochemical pathways. It is suitable for various applications, including SDS-PAGE and mass spectrometry (MS) . The protein does not bind calcium, which differentiates it from other myosin light chains .

For optimal stability, MYL6 should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it is recommended to store the protein frozen at -20°C with a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .

MYL6 is intended for laboratory research use only and should not be used as drugs, agricultural or pesticidal products, food additives, or household chemicals .