MYL4 Human

Myosin Light Chain 4 Human Recombinant
Cat. No.
BT15457
Source
E.coli.
Synonyms
Myosin light chain 4 alkali atrial embryonic, ALC1, AMLC, GT1, Myosin light chain 1 embryonic muscle/atrial isoform, Myosin light chain alkali GT-1 isoform, PRO1957, MLC1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MYL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids (1-197 a.a.) and having a molecular mass of 22.6kDa.
MYL4 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MYL4, a crucial component of muscle cells, functions as a hexameric ATPase cellular motor protein. It comprises six protein subunits: two non-phosphorylatable alkali light chains, two heavy chains, and two phosphorylatable regulatory light chains. This protein is encoded by the MYL4 gene, responsible for producing a myosin alkali light chain found in both embryonic muscle and the atria of adult hearts. Interestingly, two distinct transcript variants of this gene exist, both encoding the same protein through alternative splicing.
Description
Recombinant MYL4, specifically the human variant, is produced in E. coli bacteria. This process yields a single, non-glycosylated polypeptide chain consisting of 205 amino acids (specifically amino acids 1 through 197). The resulting protein has a molecular weight of 22.6 kDa. For purification and analytical purposes, an 8-amino acid His-tag is attached to the C-terminus of the MYL4 protein. Purification is then achieved using specialized chromatographic techniques.
Physical Appearance
The purified MYL4 solution is sterile-filtered and appears colorless.
Formulation
The MYL4 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep the MYL4 protein solution refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To preserve protein integrity during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
SDS-PAGE analysis confirms that the purity of the MYL4 protein is greater than 90%.
Synonyms
Myosin light chain 4 alkali atrial embryonic, ALC1, AMLC, GT1, Myosin light chain 1 embryonic muscle/atrial isoform, Myosin light chain alkali GT-1 isoform, PRO1957, MLC1.
Source
E.coli.
Amino Acid Sequence
MAPKKPEPKK EAAKPAPAPA PAPAPAPAPA PEAPKEPAFD PKSVKIDFTA DQIEEFKEAF SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK PKPEEMNVKM LDFETFLPIL QHISRNKEQG TYEDFVEGLR VFDKESNGTV MGAELRHVLA TLGEKMTEAE VEQLLAGQED ANGCINYEAF VKHIMSGLEH HHHHH.

Product Science Overview

Gene and Protein Structure

MYL4 is encoded by the MYLK4 gene. This gene belongs to the myosin light chain kinase family, which includes several kinases such as MYLK, MYLK2, MYLK3, and MYLK4 . The MYLK4 gene is responsible for encoding a protein that is involved in the phosphorylation of myosin light chains, a critical step in muscle contraction.

Function and Expression

MYL4 has historically been classified as a myosin of the atria and embryonic heart. It plays an important role in the contractile mechanisms of the sarcomere by increasing cross-bridge kinetics and sensitivity to calcium ions (Ca2+), which allows for greater force production . This protein is robustly expressed in the atria and is universally present in fetal and neonatal ventricular tissues .

Clinical Significance

The expression of MYL4 is associated with various cardiac conditions. For instance, higher MYL4 expression has been modestly associated with hypertrophic cardiomyopathy . Additionally, sex disparities in cardiac function have been observed, with higher MYL4 expression in male subjects . These disparities are linked to differences in protein levels, contraction times, relaxation times, and myofilament responsiveness.

Recombinant MYL4

Recombinant MYL4 refers to the protein that has been genetically engineered and produced in a laboratory setting. This recombinant form is used in various research and clinical applications to study its function and role in muscle contraction and cardiac diseases. The production of recombinant MYL4 involves inserting the MYLK4 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities.

Research and Applications

Research on MYL4 has provided valuable insights into its role in muscle contraction and cardiac function. Studies have demonstrated that MYL4 is crucial for increasing force production in the heart, making it a potential target for therapeutic interventions in cardiac diseases . Additionally, the recombinant form of MYL4 is used in various assays and experiments to understand its biochemical properties and interactions with other proteins.

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