MYL4 Antibody

Myosin Light Chain 4, Mouse Anti Human
Cat. No.
BT26878
Source
Synonyms
Myosin light chain 4 alkali atrial embryonic, ALC1, AMLC, GT1, Myosin light chain 1 embryonic muscle/atrial isoform, Myosin light chain alkali GT-1 isoform, PRO1957, MLC1.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
MYL4, a crucial component of muscle contraction, is a hexameric ATPase cellular motor protein. It comprises two nonphosphorylatable alkali light chains, two heavy chains, and two phosphorylatable regulatory light chains. The gene encoding MYL4 produces a myosin alkali light chain found in embryonic muscle and adult atria. This gene has two identified alternatively spliced transcript variants that code for the same protein.
Physical Appearance
The product is a colorless solution that has undergone sterile filtration.
Formulation
The antibody solution is provided at a concentration of 1mg/ml and is formulated in a buffer containing PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For long-term storage, it is recommended to store at -20°C. Repeated freezing and thawing of the product should be avoided.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for use in ELISA and Western blot analysis, demonstrating its specificity and reactivity. However, optimal working dilutions should be determined empirically for each specific application. As a starting point, a dilution of 1:1000 is recommended.
Synonyms
Myosin light chain 4 alkali atrial embryonic, ALC1, AMLC, GT1, Myosin light chain 1 embryonic muscle/atrial isoform, Myosin light chain alkali GT-1 isoform, PRO1957, MLC1.
Purification Method
MYL4 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4E8AT.
Immunogen
Anti-human MYL4 mAb, clone PAT4E8AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL4 protein 1-197 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Function and Importance

MYL4 is primarily found in embryonic muscle and adult atria. It plays a crucial role in muscle contraction by interacting with actin filaments and facilitating the sliding mechanism necessary for muscle movement. Unlike other myosin light chains, MYL4 does not bind calcium .

Related Pathways and Diseases

MYL4 is involved in several important biological pathways, including the activation of cAMP-dependent protein kinase A (PKA) and the regulation of the actin cytoskeleton by Rho GTPases. Mutations or dysregulation of the MYL4 gene have been associated with familial atrial fibrillation, a condition characterized by irregular heartbeats .

Mouse Anti Human MYL4 Antibody

The mouse anti-human MYL4 antibody is a polyclonal antibody raised against the full-length human MYL4 protein. This antibody is used in various research applications, including Western blotting, to detect MYL4 expression in human tissues. It is particularly useful in studying the role of MYL4 in muscle development and cardiac function .

Research Applications

Researchers utilize the mouse anti-human MYL4 antibody to investigate the molecular mechanisms underlying muscle contraction and heart function. By studying MYL4 expression and its interactions with other proteins, scientists aim to develop better treatments for conditions like atrial fibrillation and other muscle-related disorders .

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Related Products

MYL4 Human
Myosin Light Chain 4 Human Recombinant
Cat. No.
BT15457
Source
E.coli.
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