MYDGF Mouse

Myeloid Derived Growth Factor Mouse Recombinant
Cat. No.
BT9212
Source
Escherichia Coli.
Synonyms

D17Wsu104e, Il25, Ly6elg, MYDGF, Interleukin-25, IL-25, Stromal cell-derived growth factor SF20.

Appearance
Sterile Filtered clear solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MYDGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (25-166 a.a) and having a molecular mass of 18.1kDa.

MYDGF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Myeloid-derived growth factor (Mydgf) is a protein that acts locally. It is produced by bone marrow monocytes and supports the survival of cardiac muscle cells. Mydgf also encourages the growth of new blood vessels in the heart, which helps protect and repair the heart muscle after a heart attack. Mydgf promotes the growth of cells that line blood vessels by activating a specific signaling pathway involving MAPK1/3, STAT3, and CCND1. When comparing mice without Mydgf to normal mice, those lacking Mydgf develop more severe heart attack damage and weaker heart function.
Description
Recombinant MYDGF protein for mice is produced in E. coli bacteria. It is a single chain of 165 amino acids (specifically, amino acids 25 to 166) with a molecular weight of 18.1 kDa. This protein is not glycosylated, meaning it lacks attached sugar molecules. A 23 amino acid His-tag is attached to the N-terminus of the MYDGF protein to aid in purification, which is carried out using specialized chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The MYDGF protein solution has a concentration of 0.5 mg/ml and is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the MYDGF protein solution should be kept at 4°C. For longer storage, it is recommended to store the solution at -20°C. Adding a carrier protein (like HSA or BSA) at a concentration of 0.1% is advisable for long-term storage. To maintain the protein's quality, avoid repeatedly freezing and thawing the solution.
Purity
The purity of the MYDGF protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms

D17Wsu104e, Il25, Ly6elg, MYDGF, Interleukin-25, IL-25, Stromal cell-derived growth factor SF20.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSVSEPTTV PFDVRPGGVV HSFSQDVGPG NKFTCTFTYA SQGGTNEQWQ MSLGTSEDSQ HFTCTIWRPQ GKSYLYFTQF KAELRGAEIE YAMAYSKAAF ERESDVPLKS EEFEVTKTAV SHRPGAFKAE LSKLVIVAKA ARSEL.

Product Science Overview

Discovery and Nomenclature

MYDGF was first identified in bone marrow-derived monocytes and macrophages, where it was found to have cardioprotective and tissue-repairing properties, particularly after myocardial infarction . Due to its potent cardiomyocyte-protective and angiogenic activity, it was formally named Myeloid-Derived Growth Factor (MYDGF) .

Structure and Function

MYDGF is present in nearly 140 human tissues and cell lines, with the highest abundance in the oral epithelium and skin . It plays a significant role in various physiological and pathological processes, including:

  • Cell apoptosis and proliferation
  • Tissue repair and regeneration
  • Anti-inflammation
  • Glycolipid metabolism regulation
Role in Diseases

MYDGF has been implicated in several diseases, including:

  • Cardiovascular diseases: MYDGF is crucial for cardioprotection and repair after myocardial infarction .
  • Metabolic disorders: It plays a role in regulating glycolipid metabolism .
  • Renal disease: MYDGF has been shown to have protective effects in renal diseases .
  • Autoimmune/inflammatory disorders: It has anti-inflammatory properties that are beneficial in these conditions .
  • Cancers: MYDGF is involved in the regulation of cell proliferation and apoptosis, making it relevant in cancer research .
Research and Applications

Research on MYDGF is ongoing, with studies focusing on its structure, function, and mechanisms in health and disease . The recombinant form of MYDGF, particularly from mouse models, is used extensively in research to understand its therapeutic potential and underlying mechanisms.

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