MSRB2 Human
Description
MSRB2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Methionine Sulfoxide Reductase B2 (MsrB2) is an enzyme that plays a crucial role in the repair of oxidatively damaged proteins. It is part of the methionine sulfoxide reductase (Msr) system, which is responsible for reducing methionine sulfoxide (MetO) back to methionine, thereby protecting cells from oxidative stress. MsrB2 specifically reduces the R-isomer of MetO, distinguishing it from MsrA, which reduces the S-isomer .
Oxidative stress is a condition characterized by excessive reactive oxygen species (ROS) that can damage cellular components, including proteins. Methionine residues in proteins are particularly susceptible to oxidation, forming MetO. The accumulation of MetO can impair protein function and contribute to various diseases, including neurodegenerative disorders, cardiovascular diseases, and diabetes .
MsrB2 is highly expressed in mitochondria, where it plays a vital role in maintaining mitochondrial function by repairing oxidized proteins. This is particularly important in tissues with high metabolic rates, such as the heart and brain .
Research has shown that MsrB2 has protective effects against several diseases. For instance, in diabetic cardiomyopathy, a condition characterized by heart damage due to diabetes, MsrB2 helps mitigate oxidative damage and improve cardiac function. Studies in diabetic mice have demonstrated that increased expression of MsrB2 in the heart can reduce ROS levels, decrease protein oxidation, and prevent cardiac fibrosis .
Additionally, MsrB2 has been implicated in neuroprotection. In models of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, MsrB2 helps protect neurons from oxidative damage, thereby preserving cognitive and motor functions .
Human recombinant MsrB2 is produced using recombinant DNA technology, which involves inserting the human MsrB2 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes.
Recombinant MsrB2 is used in various studies to understand its function, regulation, and potential therapeutic applications. It is also employed in assays to measure the activity of the Msr system and to screen for compounds that can modulate its activity .
