MSRA E.Coli
Description
MSRA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA.
Product Science Overview
Methionine Sulfoxide Reductase A (MsrA) is an enzyme that plays a crucial role in the repair of oxidatively damaged proteins. It specifically reduces methionine sulfoxide (MetO) back to methionine, thereby protecting cells from oxidative stress. This enzyme is highly conserved across different species, including bacteria, plants, and animals. In Escherichia coli (E. coli), MsrA is essential for maintaining cellular function under oxidative stress conditions.
MsrA produced in E. coli is a single, non-glycosylated polypeptide chain containing 232 amino acids and has a molecular mass of approximately 25.4 kDa . The enzyme is fused to a 20 amino acid His-tag at the N-terminus, which facilitates its purification using chromatographic techniques . MsrA specifically reduces the S-form of methionine sulfoxide (Met-S-SO) to methionine, while another enzyme, MsrB, reduces the R-form (Met-R-SO) .
The MsrA enzyme is vital for the survival of E. coli under oxidative stress conditions. Reactive oxygen species (ROS) and hydrogen peroxide can oxidize methionine residues in proteins, leading to the formation of MetO. This modification can alter protein function and lead to the accumulation of damaged proteins. MsrA helps to reverse this damage by reducing MetO back to methionine, thereby restoring the normal function of proteins .
Recombinant MsrA is produced in E. coli using genetic engineering techniques. The gene encoding MsrA is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured under conditions that induce the expression of the MsrA protein. The recombinant protein is then purified using affinity chromatography, taking advantage of the His-tag fused to the N-terminus of the protein .
Recombinant MsrA has several applications in research and biotechnology. It is used to study the mechanisms of oxidative stress and protein repair in cells. Additionally, MsrA can be used in the development of therapeutic strategies to combat oxidative stress-related diseases. The enzyme’s ability to repair oxidatively damaged proteins makes it a valuable tool for understanding cellular responses to oxidative stress and developing interventions to mitigate its effects .
