MRPS25 Human
Mitochondrial Ribosomal Protein S25 Human Recombinant
Cat. No.
BT13768
Source
Escherichia Coli.
Synonyms
Mitochondrial Ribosomal Protein S25, Mitochondrial 28S Ribosomal Protein S25, 28S Ribosomal Protein S25 Mitochondrial, MRP-S25, RPMS25, S25mt.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MRPS25 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-173) and having a molecular mass of 22.5kDa.
MRPS25 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MRPS25 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
MRPS25, belonging to the ribosomal protein S5P family, is a 28S subunit protein. A pseudogene with the same function is found on chromosome 4.
Description
MRPS25 Human Recombinant, produced in E.coli, is a single, non-glycosylated polypeptide chain consisting of 196 amino acids (1-173) and possessing a molecular mass of 22.5kDa. It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The MRPS25 solution (concentration: 1mg/ml) is composed of 20mM Tris-HCl buffer with a pH of 8.0, 0.4M Urea, and 10% glycerol.
Stability
For use within 2-4 weeks, store at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
SDS-PAGE analysis indicates purity greater than 90%.
Synonyms
Mitochondrial Ribosomal Protein S25, Mitochondrial 28S Ribosomal Protein S25, 28S Ribosomal Protein S25 Mitochondrial, MRP-S25, RPMS25, S25mt.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPMKGRF PIRRTLQYLS QGNVVFKDSV KVMTVNYNTH GELGEGARKF VFFNIPQIQY KNPWVQIMMF KNMTPSPFLR FYLDSGEQVL VDVETKSNKE IMEHIRKILG KNEETLREEE EEKKQLSHPA NFGPRKYCLR ECICEVEGQV PCPSLVPLPK EMRGKYKAAL KADAQD
Product Science Overview
Gene and Protein Structure
Function
Clinical Significance
Mutations in the MRPS25 gene have been associated with various diseases, including Combined Oxidative Phosphorylation Deficiency 50 and Tango2-Related Metabolic Encephalopathy and Arrhythmias . These conditions highlight the importance of MRPS25 in maintaining mitochondrial function and overall cellular health.
Research and Applications
Recombinant MRPS25 protein is used in research to study mitochondrial function and the role of mitoribosomes in protein synthesis. Understanding the structure and function of MRPS25 can provide insights into mitochondrial diseases and potential therapeutic targets.
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