Monkeypox A33R
Escherichia Coli.
Sterile Filtered clear solution.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
The E.Coli derived recombinant A33R protein contains the Envelope Monkeypox immunodominant regions, having an Mw of 23kDa. The Monkeypox protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique
Product Specs
Monkeypox virus is a viral zoonotic disease transmitted from animals to humans. The virus is primarily found in rodents and primates, and it causes symptoms similar to smallpox, albeit milder. Belonging to the Orthopoxvirus genus within the Poxviridae family, monkeypox is characterized by an enveloped double-stranded DNA structure with a genome size of approximately 190 kb. Two distinct genetic clades of monkeypox exist: the central African clade, known for its higher transmissibility, and the west African clade. Transmission occurs through animal bites or direct contact with infected bodily fluids. Following an incubation period of 12 days, common symptoms such as fever, rash, headache, swollen lymph nodes, and muscle pain manifest.
The recombinant Monkeypox A33R protein, derived from E. coli, encompasses the immunodominant regions of the Monkeypox virus envelope. With a molecular weight of 23kDa, the protein is fused with a 6xHis tag at the C-terminus and purified using a proprietary chromatographic method.
The product appears as a clear, sterile-filtered solution.
The recombinant Monkeypox protein solution is formulated with PBS, 0.05% sodium nitrate, and 25mM potassium carbonate (K2CO3).
Upon receipt, the product should be stored at -20°C. To maintain product integrity, avoid repeated freeze-thaw cycles.
The purity of the protein exceeds 95% as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Escherichia Coli.
Product Science Overview
Monkeypox is a zoonotic viral disease caused by the monkeypox virus (MPXV), which belongs to the Orthopoxvirus genus of the Poxviridae family. The virus is closely related to the variola virus, which causes smallpox. Although monkeypox is less severe than smallpox, it has become a significant public health concern due to its potential to cause outbreaks.
The A33R protein is a membrane protein found in the extracellular enveloped form (EEV) of the vaccinia virus (VACV), which is a close relative of MPXV. The A33R protein plays a crucial role in the transmission of viral particles from cell to cell. It is involved in mediating the lysis of the EEV membrane, complement-mediated lysis, and T cell response . The A33R protein is highly homologous to the A35R protein of MPXV, with a homology of approximately 96.1% .
Recombinant A33R protein refers to the A33R protein that has been genetically engineered and expressed in a host system, such as Escherichia coli (E. coli). This recombinant protein can be used for various research and development purposes, including the creation of diagnostic tests, vaccines, and therapeutic drugs .
The A33R protein is a potential target for the development of vaccines against MPXV. Studies have shown that antibodies targeting the A33R protein can effectively cross-neutralize VACV, cowpox virus (CPXV), MPXV, and variola virus (VARV) . This cross-reactivity makes the A33R protein an attractive candidate for vaccine development, as it can provide broad protection against multiple Orthopoxviruses.
Research on the A33R protein has led to the development of mRNA vaccines encoding fusion proteins of MPXV antigens, including A33R. These vaccines have shown high efficacy and safety in protecting mice from lethal doses of VACV . Additionally, recombinant A33R protein can be used in serologic assays to measure antibody responses against MPXV and other Orthopoxviruses .
