MME Human
Sf9, Insect cells.
Membrane Metalloendopeptidase, Common Acute Lymphocytic Leukemia Antigen, Neutral Endopeptidase 24.11, Skin Fibroblast Elastase, Neutral Endopeptidase, Atriopeptidase, Enkephalinase, EC 3.4.24.11, Neprilysin, CALLA, NEP, SFE,Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10), Membrane Metallo-Endopeptidase Variant 1, Membrane Metallo-Endopeptidase Variant 2, Neprilysin-390, Neprilysin-411, CD10 Antigen, EC 3.4.24, CMT2T, SCA43, CD10, EPN, MME.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
MME Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 708 amino acids (52-750 a.a.) and having a molecular mass of 80.9kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
MME is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Membrane Metalloendopeptidase (MME) is a zinc-dependent enzyme found on the surface of many cell types. It plays a crucial role in breaking down various peptides, including amyloid beta, a protein implicated in Alzheimer's disease. MME is highly expressed in the kidney and is also found in other tissues. In the context of acute lymphocytic leukemia (ALL), MME serves as a vital cell surface marker for diagnosis due to its presence on early B-cell precursors and lymph node germinal centers.
This recombinant human MME protein is produced in Sf9 insect cells. It is a single, glycosylated polypeptide chain encompassing amino acids 52 to 750 of the MME protein sequence. This results in a protein with a molecular weight of 80.9 kDa. Note: On SDS-PAGE, the protein may appear between 70-100 kDa due to glycosylation. The recombinant protein is tagged with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic methods.
The MME protein solution is provided at a concentration of 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0), containing 100 mM NaCl, 0.1 mM PMSF, and 10% glycerol.
The purity of the MME protein is greater than 95%, as determined by SDS-PAGE analysis.
Membrane Metalloendopeptidase, Common Acute Lymphocytic Leukemia Antigen, Neutral Endopeptidase 24.11, Skin Fibroblast Elastase, Neutral Endopeptidase, Atriopeptidase, Enkephalinase, EC 3.4.24.11, Neprilysin, CALLA, NEP, SFE,Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10), Membrane Metallo-Endopeptidase Variant 1, Membrane Metallo-Endopeptidase Variant 2, Neprilysin-390, Neprilysin-411, CD10 Antigen, EC 3.4.24, CMT2T, SCA43, CD10, EPN, MME.
Sf9, Insect cells.
ADPYDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIP ETSSRYGNFD ILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLL KLLPDIYGWP VATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIH IDQPRLGLPS RDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVME LEKEIANATA KPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISI TNEEDVVVYA PEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKAL YGTTSETATW
RRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDL TWMDAETKKR AEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFS QSKQLKKLRE KVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGI GMVIGHEITH GFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQH LNGINTLGEN IADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTY RPEYAVNSIK TDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VWHHHHHH
Product Science Overview
Membrane Metalloendopeptidase (MME), also known as neprilysin or CD10, is a zinc-dependent metalloprotease enzyme that plays a crucial role in various physiological processes. This enzyme is encoded by the MME gene in humans and is involved in the degradation of several bioactive peptides. The recombinant form of this enzyme is often used in research to study its function and potential therapeutic applications.
MME is an integral membrane protein that is primarily found on the surface of various cell types, including epithelial cells, fibroblasts, and certain immune cells. The enzyme consists of a single polypeptide chain with a molecular weight of approximately 100 kDa. It contains a zinc-binding motif that is essential for its catalytic activity.
The primary function of MME is to degrade bioactive peptides, including enkephalins, atrial natriuretic peptide, and amyloid-beta peptides. By regulating the levels of these peptides, MME plays a critical role in maintaining homeostasis in the body. For instance, the degradation of amyloid-beta peptides by MME is thought to be protective against the development of Alzheimer’s disease .
MME is involved in several physiological and pathological processes:
- Immune Function: MME modulates immune responses by degrading peptides that can act as signaling molecules .
- Cardiovascular Health: By degrading atrial natriuretic peptide, MME helps regulate blood pressure and fluid balance .
- Neurological Health: The enzyme’s ability to degrade amyloid-beta peptides is significant in the context of neurodegenerative diseases like Alzheimer’s .
Alterations in MME expression or activity have been linked to various diseases:
- Systemic Lupus Erythematosus (SLE): Studies have shown that MME expression is positively correlated with SLE, suggesting a potential role in the disease’s pathogenesis .
- Breast Cancer: MME expression is significantly decreased in breast cancer tissues, particularly in luminal B and infiltrating ductal carcinoma subtypes. This decrease is associated with a lower risk of breast cancer in patients with SLE .
- Alzheimer’s Disease: Reduced MME activity is linked to the accumulation of amyloid-beta peptides, a hallmark of Alzheimer’s disease .
The recombinant form of MME is widely used in research to explore its function and therapeutic potential. For example, MME inhibitors are being investigated as potential treatments for hypertension and heart failure. Additionally, enhancing MME activity could be a therapeutic strategy for preventing or treating Alzheimer’s disease .
