MME Human, Active
Membrane Metalloendopeptidase, Common Acute Lymphocytic Leukemia Antigen, Neutral Endopeptidase 24.11, Skin Fibroblast Elastase, Neutral Endopeptidase, Atriopeptidase, Enkephalinase, EC 3.4.24.11, Neprilysin, CALLA, NEP, SFE,Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10), Membrane Metallo-Endopeptidase Variant 1, Membrane Metallo-Endopeptidase Variant 2, Neprilysin-390, Neprilysin-411, CD10 Antigen, EC 3.4.24, CMT2T, SCA43, CD10, EPN, MME.
Greater than 95.0% as determined by SDS-PAGE.
Description
MME Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 708 amino acids (52-750 aa) and having a molecular mass of 80.9kDa.
MME is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Neutral endopeptidase (NEP), also known as membrane metallo-endopeptidase, is an enzyme found on the surface of various cells, including lymphoid progenitors, human podocytes, and certain epithelial cells. It plays a role in breaking down biologically active proteins.
This product consists of the human form of the MME protein, produced in Sf9 insect cells using a baculovirus expression system. This protein is a single chain with glycosylation, containing 708 amino acids (specifically, amino acids 52 to 750 of the full protein sequence) and has a molecular weight of 80.9 kDa. For purification and detection purposes, a six-histidine tag is present at the C-terminus. The protein has been purified using proprietary chromatographic methods.
The MME protein is supplied in a solution at a concentration of 1 mg/ml. The solution contains 10% glycerol, 20 mM Tris-HCl buffer at a pH of 8.0, 0.1 mM PMSF (a protease inhibitor), and 100 mM NaCl (sodium chloride).
For short-term storage (up to 2-4 weeks), the solution can be kept at refrigerated temperature (4°C). For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as albumin (HSA or BSA) to a final concentration of 0.1% is advised for long-term storage. It is important to avoid repeated cycles of freezing and thawing the protein solution.
The purity of this product is greater than 95%, as determined by SDS-PAGE analysis.
The specific activity of this product is greater than 5,000 pmol/min/ug, as measured by its ability to cleave the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2. One unit of enzyme activity is defined as the amount required to convert 1.0 picomole of substrate to the fluorescent product MCA-Pro-Leu-OH per minute at a pH of 8.8 and a temperature of 25°C.
Membrane Metalloendopeptidase, Common Acute Lymphocytic Leukemia Antigen, Neutral Endopeptidase 24.11, Skin Fibroblast Elastase, Neutral Endopeptidase, Atriopeptidase, Enkephalinase, EC 3.4.24.11, Neprilysin, CALLA, NEP, SFE,Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10), Membrane Metallo-Endopeptidase Variant 1, Membrane Metallo-Endopeptidase Variant 2, Neprilysin-390, Neprilysin-411, CD10 Antigen, EC 3.4.24, CMT2T, SCA43, CD10, EPN, MME.
ADPYDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIP ETSSRYGNFD ILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLL KLLPDIYGWP VATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIH IDQPRLGLPS RDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVME LEKEIANATA KPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISIT NEEDVVVYAP EYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKAL YGTTSETATW RRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDL TWMDAETKKR AEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFS QSKQLKKLRE KVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGI GMVIGHEITH GFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQH LNGINTLGEN IADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTY RPEYAVNSIK TDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VWHHHHHH
Product Science Overview
MME is involved in the degradation of several bioactive peptides, including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin . It cleaves peptides at the amino side of hydrophobic residues, thereby inactivating these peptide hormones . This enzyme is particularly abundant in the kidney and is also expressed in a wide variety of tissues .
MME has been identified as a tumor suppressor in various cancers, such as prostate carcinogenesis and esophageal squamous cell carcinoma . Its expression is usually downregulated in tumor tissues, and it serves as a valuable diagnostic biomarker for certain cancers . For instance, in breast cancer (BRCA), MME expression is significantly decreased, especially in luminal B and infiltrating ductal carcinoma subtypes .
Moreover, MME is positively correlated with systemic lupus erythematosus (SLE) and may inhibit the occurrence of breast cancer in SLE patients via the PI3K/AKT/FOXO signaling pathway . This dual role highlights its importance in both cancer biology and autoimmune diseases.
Recombinant MME is produced using recombinant DNA technology, which involves inserting the MME gene into a suitable expression system, such as bacteria or mammalian cells, to produce the active enzyme. This recombinant form is used in various research and clinical applications to study its function and potential therapeutic uses.
MME is a common acute lymphocytic leukemia antigen (CALLA) and is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL) . It is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL . Additionally, MME is used in the diagnosis of other hematologic diseases, including angioimmunoblastic T cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma .
