MIP 5 (68 a.a) Human

CCL15 is a single, non-glycosylated polypeptide chain consisting of 68 amino acids . The protein has a molecular mass of approximately 7.4 kDa . The amino acid sequence of CCL15 is as follows:

SFHFAADCCT SYISQSIPCS LMKSYFETSS ECSKPGVIFL TKKGRQVCAK PSGPGVQDCM KKLKPYSI

The gene encoding CCL15 is located on chromosome 17, where many human CC chemokine genes are found . CCL15 shares 35% amino acid homology with another human chemokine, CCL14 (HCC1) . It is most abundantly expressed in the heart, skeletal muscle, and adrenal gland, with lower expression levels in the liver, small intestine, colon, and certain leukocytes and macrophages in the lung .

CCL15 is fully biologically active and has been shown to be chemotactic for T cells and monocytes . It induces calcium flux in human CCR-1-transfected cells . The biological activity of CCL15 is typically measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells, with an effective dose (ED50) in the range of 2-4 ng/ml .

Recombinant human CCL15 is produced in Escherichia coli (E. coli) and purified using high-performance liquid chromatography (HPLC) and other chromatographic techniques . The protein is lyophilized from a filtered concentrated solution containing phosphate-buffered saline (PBS) at pH 7.4 . It is recommended to reconstitute the lyophilized protein in sterile water or an aqueous buffer containing 0.1% bovine serum albumin (BSA) to a concentration of 0.1-1.0 mg/mL .

Lyophilized CCL15 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, the protein should be stored at 4°C for 2-7 days and below -18°C for future use . To ensure maximal stability, it is recommended to add a carrier protein (0.1% HSA or BSA) and avoid repeated freeze-thaw cycles .

CCL15 is used in various research applications, including studies on immune cell migration, inflammation, and chemokine signaling pathways. It is also utilized in bioassays to evaluate its chemotactic properties and biological activity .