MIP 5 Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The MIP5 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CCL15 is a non-glycosylated polypeptide chain consisting of 92 amino acids and has a molecular mass of approximately 10.1 kDa . The protein is produced in E. coli and is purified using proprietary chromatographic techniques . The amino acid sequence of CCL15 includes a series of conserved cysteine residues that are characteristic of the CC chemokine family .
The gene encoding CCL15 is located on chromosome 17 in humans, within a cluster of similar genes . CCL15 shares about 35% amino acid homology with another chemokine, CCL14 (HCC1) . The expression of CCL15 is most abundant in the heart, skeletal muscle, and adrenal gland, with lower levels found in the liver, small intestine, colon, and certain leukocytes and macrophages of the lung .
CCL15 is known for its ability to chemoattract human T-lymphocytes and monocytes . It acts through the C-C chemokine receptor type 1 (CCR1) . The biological activity of CCL15 is determined by its ability to induce chemotaxis in human T-lymphocytes at concentrations ranging from 1-10 ng/ml, corresponding to a specific activity of 100,000-1,000,000 IU/mg .
Lyophilized CCL15 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term stability . Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage . To prevent degradation, it is recommended to add a carrier protein such as 0.1% HSA or BSA and avoid repeated freeze-thaw cycles .
CCL15 is used in various research applications, including studies on immune response, inflammation, and cell signaling. Its ability to attract T-lymphocytes and monocytes makes it a valuable tool for investigating the mechanisms of immune cell migration and the role of chemokines in disease processes.
