MIF Human His N
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
MIF human Recombinant, fused to 40 a.a. His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Macrophage Inducing Factor Human Recombinant ( 1-115 a.a. ) is a single, non-glycosylated, polypeptide chain having a total amino acids of 155 and molecular mass of 17kDa.
Product Specs
Product Science Overview
Macrophage Migration Inhibitory Factor (MIF) is a critical cytokine involved in the regulation of the immune response. It was one of the first cytokines to be discovered and has since been recognized for its role in various physiological and pathological processes. The recombinant form of human MIF, tagged with a His-tag at the N-terminus, is widely used in research to study its functions and interactions.
The recombinant human MIF is typically expressed in insect cells and purified to high homogeneity. It is a 15 kDa protein consisting of 124 amino acid residues, including an N-terminal His-tag . The His-tag facilitates purification and detection of the protein. The protein is lyophilized and can be reconstituted in water for experimental use .
MIF plays a multifaceted role in the immune system. It acts as a pro-inflammatory cytokine and an enzyme. MIF’s pro-inflammatory activity is mediated through its interaction with receptors such as CD74 and CD44, leading to the secretion of various cytokines like TNF-α, IL-1, IL-6, and IL-8 . Additionally, MIF has enzymatic activity, functioning as a tautomerase that catalyzes the keto-enol isomerization of substrates like phenylpyruvate and L-dopachrome .
MIF is released from immune cells in response to glucocorticoids, counteracting their immunosuppressive effects. This counter-regulatory mechanism is crucial for maintaining immune homeostasis. MIF also inhibits the random migration of macrophages, hence its name . The protein’s enzymatic activity is dependent on its trimeric configuration and the presence of a free N-terminal proline residue .
Recombinant human MIF with an N-terminal His-tag is extensively used in research to study its biological functions and interactions. It is employed in various assays to investigate its role in inflammation, immune response, and disease pathogenesis. The His-tag allows for easy purification and detection, making it a valuable tool in molecular biology and biochemistry .
