MIF Mouse, His

Macrophage Migration Inhibitory Factor (MIF) is a multifunctional protein that plays a crucial role in the immune system. It is involved in various biological processes, including inflammation, immune response, and cell proliferation. The recombinant form of MIF, particularly the mouse variant with a His tag, is widely used in research to study its functions and mechanisms.

MIF is a pro-inflammatory cytokine that is secreted by various cells, including macrophages and T cells. It has a unique ability to counteract the anti-inflammatory effects of glucocorticoids, making it a critical player in the immune response . Structurally, MIF assembles into a trimer composed of three identical subunits, each containing two antiparallel alpha helices and a four-stranded beta sheet .

The recombinant form of MIF is produced using genetic engineering techniques. The mouse variant is often tagged with a His (histidine) tag to facilitate purification and detection. This recombinant protein is expressed in various systems, including insect cells, to ensure proper folding and functionality . The His tag allows for easy purification using nickel affinity chromatography, making it a valuable tool for researchers.

MIF is involved in several key biological processes:

• Immune Response: MIF is released in response to bacterial antigens and interacts with its receptor, CD74, on immune cells to trigger an acute immune response .
• Inflammation: It plays a significant role in promoting inflammation by counteracting the effects of glucocorticoids .
• Cell Proliferation and Survival: MIF-CD74 signaling activates pathways that promote cell survival and proliferation, especially during injury .

Recombinant MIF is extensively used in research to study its role in various diseases, including autoimmune disorders, cancer, and infectious diseases. The availability of the mouse recombinant form with a His tag allows for detailed investigations into its structure, function, and interactions with other proteins .