MIA Human, His

Melanoma Inhibitory Activity Protein Human Recombinant, His Tag
Cat. No.
BT23637
Source
Escherichia Coli.
Synonyms
Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MIA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 128 amino acids (25-131) and having a molecular mass of 14.4 kDa.
MIA is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
Melanoma Inhibitory protein (MIA) is a protein that has been shown to inhibit the growth of melanoma cells in vitro. MIA contains a SH3 domain and acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. Studies have shown that MIA mRNA expression is inversely correlated with pigmentation in human melanoma cell lines with different metastatic capacities. MIA has been shown to be a sensitive and specific serum marker for systemic malignant melanoma and may be useful for staging primary melanomas, detecting the progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.
Description
Recombinant human MIA protein produced in E. coli is a single, non-glycosylated polypeptide chain containing 128 amino acids (25-131). The protein has a molecular mass of 14.4 kDa and is fused to a 23 amino acid His-tag at the N-terminus.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The MIA solution (0.25 mg/mL) is supplied in 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Product Science Overview

Introduction

Melanoma Inhibitory Activity (MIA) protein is a small, secreted protein that plays a significant role in the progression and metastasis of malignant melanoma. It is also known as melanoma-derived growth regulatory protein. The recombinant form of this protein, tagged with a His (histidine) tag, is widely used in research to study its structure, function, and potential therapeutic applications.

Structure and Expression

The human recombinant MIA protein with a His tag is typically expressed in systems such as HEK 293 cells or Escherichia coli. The His tag, usually consisting of six histidine residues, is added to the N-terminus of the protein to facilitate purification using metal affinity chromatography. The recombinant protein is often produced with high purity, exceeding 95%, and has a molecular weight of approximately 14.23 kDa .

Biological Function

MIA protein is involved in the regulation of cell adhesion and migration, which are critical processes in cancer metastasis. It interacts with extracellular matrix components such as fibronectin, disrupting cell-matrix interactions and promoting the detachment of melanoma cells from the primary tumor site . This detachment is a key step in the metastatic spread of melanoma cells to distant organs.

Clinical Significance

MIA is a valuable biomarker for malignant melanoma. Elevated levels of MIA in the blood are associated with advanced stages of melanoma, particularly metastatic stages III and IV . Monitoring MIA levels can aid in the diagnosis and prognosis of melanoma, as well as in assessing the response to therapy.

Research Applications

The recombinant human MIA protein with a His tag is used in various research applications, including:

  • Structural studies: Understanding the three-dimensional structure of MIA protein using techniques like NMR spectroscopy .
  • Functional assays: Investigating the biological activity of MIA in cell adhesion and migration assays.
  • Drug development: Screening for potential inhibitors of MIA to develop new therapeutic strategies for melanoma.

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THE BIOTEK
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