METAP1D Human

MAP1D is localized in the mitochondria and plays a crucial role in the N-terminal methionine excision pathway . This pathway involves the removal of the N-terminal methionine from many proteins, facilitating subsequent protein modifications . The enzyme requires the deformylation of the N(alpha)-formylated initiator methionine before it can hydrolyze the methionine .

The activity of MAP1D is essential for cell growth and viability . It has been observed that MAP1D is over-expressed in colon cancer cell lines and colon tumors, suggesting a potential role in tumorigenesis . The enzyme’s ability to remove methionine from nascent proteins is critical for maintaining cellular homeostasis and function .

Recombinant Human Methionine Aminopeptidase 1D is produced using baculovirus expression systems in Spodoptera frugiperda (Sf 21) cells . The recombinant protein typically includes an N-terminal methionine and a C-terminal 10-His tag for purification purposes . The protein is supplied as a 0.2 μm filtered solution in Tris, NaCl, and Glycerol, and is shipped with polar packs to maintain stability .

Recombinant MAP1D is used in various research applications, including studies on protein modification, enzyme activity assays, and cancer research . Its ability to remove methionine from a fluorogenic peptide substrate makes it a valuable tool for biochemical assays .

The recombinant protein should be stored at -20 to -70 °C to maintain its stability and activity . It is recommended to use a manual defrost freezer and avoid repeated freeze-thaw cycles to preserve the protein’s integrity .