Aminopeptidase

Aminopeptidase Aeromonas Recombinant is a 29 kDa protein that consists of 291 amino acid residues . It is typically expressed in Escherichia coli (E. coli) systems, which allows for efficient production and purification . The enzyme is highly purified, with a purity of ≥ 98% as determined by SDS-PAGE gel and HPLC analyses .

This enzyme functions as an exopeptidase, meaning it cleaves amino acids from the N-terminus of peptide chains . It specifically recognizes a stop sign at -X-Pro and requires a free α-amino group in the L-configuration . This makes it particularly useful for removing redundant N-terminal methionine residues that are often added during the production of recombinant proteins .

Aminopeptidase Aeromonas Recombinant has several important applications in the field of biochemistry and molecular biology:

1. Processing of Pharmaceutical Proteins: It is used in the processing of pharmaceutical proteins produced by genetic engineering .
2. Physical and Structural Investigations: The enzyme is employed in studies involving the physical and structural properties of proteins .
3. Sequence and Amino-Terminal Determinations: It aids in determining the sequence and amino-terminal of proteins .

The enzyme is typically supplied in a lyophilized form and should be stored at -20°C to maintain its stability and activity . It is shipped at ambient temperature and should be reconstituted according to the specific instructions provided with the product .