Introduction
Mediator of ErbB2-driven cell motility 1 (MEMO1), a member of the UPF0103 family, plays a crucial role in cell migration. It functions by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Moreover, MEMO1 governs the cellular localization of APC and CLASP2, two proteins involved in microtubule dynamics, by modulating GSK3B activity. Its importance in breast carcinoma cell migration suggests a potential role in tumor development. MEMO1 also participates in ERBB2 signaling pathways.
Description
Recombinant human MEMO1, expressed in E. coli, is a non-glycosylated polypeptide chain. It consists of 322 amino acids, with residues 1 to 297 representing the MEMO1 sequence, and has a molecular weight of 36.4 kDa. A 25-amino acid His-tag is fused to the N-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The MEMO1 protein solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 50% glycerol, 5 mM DTT, 300 mM NaCl, and 2 mM EDTA.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Protein MEMO1, C21orf19-like protein, Hepatitis C virus NS5A-transactivated protein 7, HCV NS5A-transactivated protein 7, Mediator of ErbB2-driven cell motility 1, Mediator of cell motility 1, Memo-1, MEMO1, C2orf4, MEMO, NS5ATP7, CGI-27.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMSNRV VCREASHAGS WYTASGPQLN AQLEGWLSQV QSTKRPARAI IAPHAGYTYC GSCAAHAYKQ VDPSITRRIF ILGPSHHVPL SRCALSSVDI YRTPLYDLRI DQKIYGELWK TGMFERMSLQ TDEDEHSIEM HLPYTAKAME SHKDEFTIIP VLVGALSESK EQEFGKLFSK YLADPSNLFV VSSDFCHWGQ RFRYSYYDES QGEIYRSIEH LDKMGMSIIE QLDPVSFSNY LKKYHNTICG RHPIGVLLNA ITELQKNGMN MSFSFLNYAQ SSQCRNWQDS SVSYAAGALT VH.