MDP1 Human

The MDP1 gene is located on chromosome 14 and is responsible for encoding the MDP1 protein . The human recombinant version of MDP1 is produced in E. coli and is a single, non-glycosylated polypeptide chain consisting of 200 amino acids . The protein has a molecular mass of approximately 22.7 kDa .

MDP1 is a magnesium-dependent enzyme, meaning it requires magnesium ions to function properly . It exhibits phosphatase activity, specifically acting on tyrosine residues . This activity is essential for regulating various cellular processes, including signal transduction and metabolic pathways.

One of the key roles of MDP1 is in the fructosamine metabolic process, where it dephosphorylates fructosamine-6-phosphate . This function is vital for maintaining cellular homeostasis and proper metabolic function.

The recombinant MDP1 is expressed in E. coli and purified using proprietary chromatographic techniques to achieve a purity greater than 95% as determined by SDS-PAGE . The protein is typically formulated in a buffer containing Tris-HCl, glycerol, DTT, and NaCl to ensure stability and activity .

MDP1 is widely used in biochemical and biomedical research to study its role in cellular processes and its potential implications in various diseases. Understanding the function and regulation of MDP1 can provide insights into metabolic disorders and other conditions where phosphatase activity is disrupted.

For optimal stability, MDP1 should be stored desiccated below -18°C. It is recommended to add a carrier protein, such as HSA or BSA, for long-term storage to prevent freeze-thaw cycles .