MCP 2 Mouse

Recombinant mouse MCP-2 is a non-glycosylated protein consisting of 74 amino acids and has a molecular mass of approximately 8.5 kDa . The protein is typically produced in E. coli and is available as a sterile, lyophilized (freeze-dried) powder . Upon reconstitution, it is recommended to use sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions .

MCP-2/CCL8 is known for its ability to activate and attract various immune cells, including mast cells, eosinophils, and basophils, which are implicated in allergic responses . It signals through several G protein-coupled receptors, including CCR1, CCR2B, and CCR5 . The activity of MCP-2 is determined by its ability to chemoattract human peripheral blood mononuclear cells (PBMCs) at concentrations ranging from 10 to 100 ng/ml .

MCP-2/CCL8 plays a pivotal role in the control of leukocyte chemotaxis, which is essential for the immune response to infections and inflammation . It is also a potent inhibitor of HIV-1 due to its high-affinity binding to the receptor CCR5, one of the major co-receptors for HIV-1 . Additionally, MCP-2 is involved in various biological processes, including the G protein-coupled receptor signaling pathway, chemokine-mediated signaling pathway, and cellular responses to tumor necrosis factor and interferon-gamma .

Recombinant mouse MCP-2 is primarily used for research purposes, including studies on immune cell chemotaxis, allergic responses, and HIV-1 inhibition . It is not intended for human, animal, or diagnostic applications .

The lyophilized product is stable at -20°C, and reconstituted material should be aliquoted and frozen at -20°C for long-term storage . It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), for extended storage .