MCP 2 Canine

Monocyte Chemotactic Protein-2 Canine Recombinant (CCL8)
Cat. No.
BT17472
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, CCL8, MCP2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MCP2 Canine Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.8kDa.
The CCL8 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-C motif) ligand 8, also known as CCL8, is a small cytokine classified as a CC chemokine. It was previously referred to as monocyte chemotactic protein-2 (MCP-2). CCL8 is initially produced as a precursor molecule consisting of 109 amino acids. This precursor undergoes cleavage to generate the mature CCL8 protein, which comprises 75 amino acids. The gene encoding CCL8 is composed of 3 exons and is situated within a large cluster of CC chemokine genes on chromosome 17q11.2 in humans. CCL8 exhibits chemotactic and activating properties towards a diverse range of immune cells. These include mast cells, eosinophils, and basophils, which play roles in allergic responses. Additionally, CCL8 acts on monocytes, T cells, and NK cells, all of which are involved in inflammatory processes. The effects of CCL8 are mediated through its binding to various cell surface receptors known as chemokine receptors. Notable among these receptors are CCR1, CCR2B, and CCR5.
Description
Recombinant Canine MCP2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 76 amino acids. It has a molecular mass of 8.8 kDa. The purification of CCL8 is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The MCP-2 protein was lyophilized from a 0.2 µm filtered solution concentrated in phosphate-buffered saline (PBS) at a pH of 7.4.
Solubility
To reconstitute the lyophilized MCP2, it is advised to dissolve it in sterile 18 MΩ·cm H₂O at a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized MCP-2, while stable at room temperature for a period of 3 weeks, should ideally be stored in a desiccated state below -18°C. Once reconstituted, CCL8 can be stored at 4°C for 2 to 7 days. For extended storage, it is recommended to store it below -18°C. To ensure long-term stability during storage, the addition of a carrier protein such as 0.1% HSA or BSA is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 98.0%, as determined by the following methods: (a) Analysis by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). (b) Analysis by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity, as assessed by a chemotaxis bioassay employing human peripheral blood monocytes, is observed within a concentration range of 10-100 ng/ml.
Synonyms
C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, CCL8, MCP2.
Source
Escherichia Coli.
Amino Acid Sequence
QPDSVSIPIT CCFSMVKRKI PMQKLESYMR ITNSQCPQEA VIFKTKASRE ICADPKQKWV QDYMNHLDQK SQAQKP.

Product Science Overview

Introduction

Monocyte Chemotactic Protein-2 (MCP-2), also known as CCL8, is a member of the C-C chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells, and they play a crucial role in immune responses by directing the migration of immune cells to sites of inflammation or injury. MCP-2/CCL8 is particularly known for its ability to attract monocytes, a type of white blood cell, to areas where they are needed.

Structure and Gene Encoding

The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce the mature CCL8 containing 75 amino acids . The gene for CCL8 is encoded by three exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans . This gene organization is conserved across various species, including canines.

Function and Signaling

MCP-2/CCL8 is implicated in allergic responses through its ability to activate mast cells, eosinophils, and basophils . It signals through several G protein-coupled receptors, including CCR1, CCR2B, and CCR5 . These receptors are expressed on the surface of various immune cells, and their activation leads to a cascade of intracellular events that result in cell migration and activation.

Role in Disease and Therapeutic Potential

The ability of MCP-2/CCL8 to attract monocytes and other immune cells makes it a key player in inflammatory diseases. Elevated levels of CCL8 have been observed in conditions such as asthma, rheumatoid arthritis, and certain infections. Understanding the role of CCL8 in these diseases can help in developing targeted therapies that modulate its activity.

Recombinant CCL8

Recombinant CCL8 is produced using genetic engineering techniques where the CCL8 gene is inserted into an expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used in research to study its function and in developing potential therapeutic applications.

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