MCP 3 Mouse
Monocyte Chemotactic Protein-3 Mouse Recombinant (CCL7)
Cat. No.
BT17730
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A7, CCL7, Monocyte chemotactic protein 3, MCP-3, Monocyte chemoattractant protein 3, NC28, chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, SCYA6, SCYA7, MGC138463, MGC138465.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Monocyte Chemotactic Protein-3 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.
The MCP-3 is purified by proprietary chromatographic techniques.
The MCP-3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-C motif) ligand 7, also known as CCL7 and formerly known as monocyte-specific chemokine 3 (MCP3), is a small cytokine belonging to the chemokine family. As a CC chemokine, CCL7 is characterized by two adjacent N-terminal cysteine residues in its mature protein. This chemokine exhibits specific chemoattractant properties towards monocytes and plays a crucial role in regulating macrophage function. CCL7 is produced by specific tumor cell lines and macrophages. In humans, the gene encoding CCL7 is located on chromosome 17 within a large cluster containing numerous other CC chemokines. Notably, CCL7 shares close proximity and homology with CCL2 (previously designated as MCP1).
Description
Recombinant Mouse Monocyte Chemotactic Protein-3, produced in E. coli, is a non-glycosylated polypeptide chain composed of 74 amino acids. With a molecular weight of 8.51 kDa, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, lyophilized powder with a white appearance.
Formulation
The protein was lyophilized from a sterile solution at a concentration of 1 mg/ml, without the addition of any extraneous additives.
Solubility
To reconstitute the lyophilized Monocyte Chemotactic Protein-3, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a minimum concentration of 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized MCP-3 demonstrates stability at room temperature for a period of 3 weeks. However, for optimal long-term storage, it is advisable to store the desiccated product at temperatures below -18°C. Once reconstituted, CCL7 should be stored at 4°C for a duration of 2-7 days. For extended storage, freezing at temperatures below -18°C is recommended. To preserve protein integrity during long-term storage, the addition of a carrier protein, such as 0.1% HSA or BSA, is advised. It is crucial to avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of this product exceeds 98.0%, as determined by the following methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of Mouse MCP-3 is evaluated based on its capacity to induce chemotaxis in Balb/C mouse spleen mononuclear cells (MNCs). At concentrations ranging from 10 to 100 ng/ml, Mouse MCP-3 exhibits a specific activity corresponding to 10,000-100,000 IU/mg.
Synonyms
Small inducible cytokine A7, CCL7, Monocyte chemotactic protein 3, MCP-3, Monocyte chemoattractant protein 3, NC28, chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, SCYA6, SCYA7, MGC138463, MGC138465.
Source
Escherichia Coli.
Amino Acid Sequence
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP.
Product Science Overview
Introduction
Monocyte Chemotactic Protein-3 (MCP-3), also known as CCL7, is a small cytokine belonging to the CC chemokine family. It plays a crucial role in the immune system by attracting monocytes, a type of white blood cell, to sites of inflammation or injury. MCP-3 is produced by macrophages and certain tumor cell lines and is involved in regulating macrophage function .
Gene and Protein Structure
Biological Functions
MCP-3 is involved in various biological processes, including:
- Monocyte Chemotaxis: MCP-3 attracts monocytes to sites of inflammation, aiding in the immune response.
- Regulation of Macrophage Function: MCP-3 can modulate the activity of macrophages, which are essential for phagocytosis and antigen presentation.
- Chemokine-Mediated Signaling Pathway: MCP-3 participates in signaling pathways that regulate immune cell migration and activation .
Production and Recombinant Forms
Recombinant MCP-3 can be produced using various expression systems, including E. coli. The recombinant protein is typically non-glycosylated and has a molecular weight of approximately 9 kDa. It is available in a lyophilized (freeze-dried) form and can be reconstituted with sterile water for research purposes .
Applications in Research
MCP-3 is widely used in research to study its role in immune responses and its potential therapeutic applications. It is particularly valuable in experiments involving:
- Cell Migration Assays: MCP-3 is used to study the chemotactic behavior of monocytes and other immune cells.
- Inflammation Models: MCP-3 is employed in models of inflammation to investigate its effects on immune cell recruitment and activation.
- Cancer Research: MCP-3’s role in tumor cell lines makes it a subject of interest in cancer research, particularly in understanding how tumors manipulate the immune system .
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