MCP 3 Human
Monocyte Chemotactic Protein-3 Human Recombinant (CCL7)
Cat. No.
BT17685
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A7, CCL7, Monocyte chemotactic protein 3, MCP-3, Monocyte chemoattractant protein 3, NC28, chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, SCYA6, SCYA7, MGC138463, MGC138465.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Monocyte Chemotactic Protein-3 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 9011 Dalton.
The MCP-3 is purified by proprietary chromatographic techniques.
The MCP-3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-C motif) ligand 7, also known as CCL7, is a small cytokine belonging to the chemokine family. Previously referred to as monocyte-specific chemokine 3 (MCP3), CCL7 is classified as a CC chemokine due to the presence of two adjacent N-terminal cysteine residues in its mature protein structure. This chemokine exhibits a specific attraction towards monocytes and plays a role in regulating macrophage function. Production of CCL7 is observed in specific tumor cell lines and macrophages. In humans, the gene encoding this chemokine is located on chromosome 17 within a large cluster that also contains numerous other CC chemokines. Notably, CCL7 shares a close relationship with CCL2, formerly known as MCP1.
Description
Recombinant Human Monocyte Chemotactic Protein-3, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 76 amino acids. With a molecular mass of 9011 Daltons, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized powder, sterile-filtered.
Formulation
The protein was lyophilized from a sterile solution at a concentration of 1 mg/ml, without the addition of any additives.
Solubility
For reconstitution of the lyophilized Monocyte Chemotactic Protein-3, it is recommended to use sterile 18 MΩ-cm H2O at a minimum concentration of 100 µg/ml. Further dilutions can be prepared using other aqueous solutions.
Stability
Lyophilized MCP-3 remains stable at room temperature for a period of 3 weeks. However, it is advisable to store it in a desiccated state below -18°C. After reconstitution, CCL7 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store it below -18°C after adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 98.0% through the following analyses:
(a) RP-HPLC analysis
(b) SDS-PAGE analysis
Biological Activity
The specific activity of MCP-3 is assessed by its capacity to chemoattract human peripheral blood. This activity is observed at concentrations ranging from 8 to 80 ng/ml, corresponding to a specific activity of 12,500-125,000 IU/mg.
Synonyms
Small inducible cytokine A7, CCL7, Monocyte chemotactic protein 3, MCP-3, Monocyte chemoattractant protein 3, NC28, chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, SCYA6, SCYA7, MGC138463, MGC138465.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.
Product Science Overview
Structure and Classification
Genetic Location
Receptors and Signaling
Biological Activity
The primary function of CCL7 is to attract monocytes to sites of inflammation or injury, where they can differentiate into macrophages and dendritic cells . This process is essential for the immune system’s ability to respond to infections and tissue damage . The biological activity of CCL7 is typically measured by its ability to chemoattract human neutrophils .
Applications and Stability
Recombinant human MCP-3 is used extensively in research to study its role in immune responses and its potential therapeutic applications . The protein is typically produced in Escherichia coli (E. coli) and is available in a lyophilized (freeze-dried) form . For long-term storage, it is recommended to keep the lyophilized product at -20°C and reconstituted material should be aliquoted and frozen at -20°C with a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) to ensure stability .
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