MCM7 Antibody
Minichromosome Maintenance Complex Component 7, MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 7, DNA Replication Licensing Factor MCM7, Homolog of S. Cerevisiae Cdc47, CDC47 Homolog, P1CDC47, PNAS146, P85MCM, MCM2, CDC47, P1.1-MCM3, EC 3.6.4.12.
Description
Product Specs
MCM7, a mini-chromosome maintenance protein (MCM), is essential for initiating eukaryotic genome replication. MCM proteins assemble into a hexameric complex, a critical part of the pre-replication complex (pre-RC), involved in replication fork formation and recruiting DNA replication proteins. The MCM complex, consisting of MCM2, 4, 6, and 7 proteins, exhibits DNA helicase activity, including DNA unwinding.
The product is provided at a concentration of 1mg/ml and is prepared in a solution containing PBS with a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
The MCM7 antibody has undergone rigorous testing using ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal results may vary depending on the specific application, and therefore, it is recommended to titrate the reagent for each individual experiment.
Minichromosome Maintenance Complex Component 7, MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 7, DNA Replication Licensing Factor MCM7, Homolog of S. Cerevisiae Cdc47, CDC47 Homolog, P1CDC47, PNAS146, P85MCM, MCM2, CDC47, P1.1-MCM3, EC 3.6.4.12.
MCM7 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT118H7AT.
Anti-human MCM7 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MCM7 protein 1-414 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
The Minichromosome Maintenance Complex Component 7 (MCM7) is a crucial protein involved in the initiation and regulation of DNA replication. It is part of the minichromosome maintenance (MCM) protein family, which plays a significant role in ensuring the proper replication of DNA during the cell cycle. The MCM complex, including MCM7, is essential for the G1/S phase transition and the maintenance of genomic stability.
MCM7 is a component of the MCM2-7 complex, which functions as a DNA helicase. This complex unwinds the DNA helix, allowing replication machinery to access the DNA strands for replication. The MCM2-7 complex is composed of six related proteins (MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7), and each component is vital for the helicase activity of the complex .
MCM7 specifically has two isoforms:
- Isoform 1: Consists of 719 amino acids.
- Isoform 2: Consists of 543 amino acids.
Both isoforms contain a conserved MCM domain in the C-terminal region, which is crucial for their function .
The Mouse Anti Human MCM7 Antibody is a monoclonal antibody produced in mice and is used for various research applications, including ELISA and Western Blot. This antibody is purified from mouse ascitic fluids by protein-A affinity chromatography and is supplied as a sterile filtered colorless solution containing PBS, glycerol, and sodium azide .
