MCM7 PAT1G8AT Antibody
Minichromosome Maintenance Complex Component 7, MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 7, DNA Replication Licensing Factor MCM7, Homolog of S. Cerevisiae Cdc47, CDC47 Homolog, P1CDC47, PNAS146, P85MCM, MCM2, CDC47, P1.1-MCM3, EC 3.6.4.12.
Description
Product Specs
Minichromosome Maintenance Complex Component 7, MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 7, DNA Replication Licensing Factor MCM7, Homolog of S. Cerevisiae Cdc47, CDC47 Homolog, P1CDC47, PNAS146, P85MCM, MCM2, CDC47, P1.1-MCM3, EC 3.6.4.12.
MCM7 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
PAT1G8AT.
Anti-human MCM7 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MCM7 protein 1-414 amino acids purified from E. coli.
Mouse IgG2b heavy chain and k light chain.
Product Science Overview
Minichromosome Maintenance Complex Component 7 (MCM7) is a crucial protein involved in the initiation and elongation of DNA replication in eukaryotic cells. It is part of the MCM2-7 complex, which forms a hexameric ring essential for the replication licensing process. This article delves into the background of MCM7, its role in DNA replication, and the specifics of the Clone PAT1G8AT, a mouse anti-human monoclonal antibody targeting MCM7.
The MCM complex is a DNA helicase necessary for genomic DNA replication. It consists of six gene products, MCM2-7, which form a heterohexamer . This complex is critical for cell division and is regulated by various checkpoint pathways, such as the S-phase entry and arrest checkpoints . The MCM complex’s loading and activation are tightly controlled and linked to cell growth cycles .
MCM7, encoded by the MCM7 gene, is one of the highly conserved mini-chromosome maintenance proteins essential for the initiation of eukaryotic genome replication . The MCM complex, including MCM7, is a key component of the pre-replication complex (pre_RC) and is involved in forming replication forks and recruiting other DNA replication-related proteins . The complex possesses DNA helicase activity, which is crucial for unwinding DNA during replication .
Clone PAT1G8AT is a monoclonal antibody derived from mouse that specifically targets human MCM7. This antibody is produced by hybridizing mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MCM7 protein . The antibody is purified from mouse ascitic fluids using protein-G affinity chromatography and is available in various quantities for research purposes .
The Clone PAT1G8AT antibody has been tested for specificity and reactivity through ELISA and Western blot analysis . It is supplied as a sterile filtered colorless solution containing PBS, pH-7.4, and 0.02% Sodium Azide . For optimal storage, it should be kept at 4°C for short-term use and at -20°C for long-term storage, avoiding freeze-thaw cycles .
