MBP Mouse Antibody

Maltose-Binding protein, Antibody
Cat. No.
BT24642
Source
Synonyms
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Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Maltose Binding Protein (MBP) is a key component of the maltose/maltodextrin system in Escherichia coli, responsible for the uptake and breakdown of maltodextrins. This intricate system comprises numerous proteins and protein complexes. MalE, also known as MBP, specifically binds to maltodextrins after they diffuse through the LamB pore in the outer membrane and enter the periplasm. This binding prevents the sugar from diffusing back out. Subsequently, MalE transfers the sugar to a complex of MalF and MalG located in the cytoplasmic membrane. Here, the ATPase MalK facilitates active transport. With a molecular weight of 40,622 daltons, MBP consists of two domains with similar secondary structures, each characterized by central pleated sheets surrounded by helices. These domains are connected by three bridges, forming a deep groove where the sugar binds. All hydroxyl groups of the sugar establish hydrogen bonds with the protein's side chains, with water molecules also participating in this network.
Applications
**Immunofluorescence:** For optimal results on frozen sections, use a dilution of 1:200 and incubate with the primary antibody for 60 minutes at 25°C. **Western Blotting:** A working dilution of 1:500 is recommended for Western blotting. Incubate with the primary antibody for 60 minutes at 25°C.
Stability
To maintain optimal performance, store the unopened antibody at a temperature of 4°C or lower. Under these conditions, the product is expected to remain stable for one year from the purchase date. Once reconstituted, the antibody should be stored at 4°C and is stable for a minimum of two months. For long-term storage, freezing aliquots of the antibody at -20°C is recommended. However, avoid using frost-free freezers for this purpose. Repeated cycles of freezing and thawing should be avoided. It is best practice to prepare working dilutions fresh on the day of use.
Specificity
Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).
Type
Mouse Antibody Monoclonal.
Immunogen
The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.
Storage Buffer
Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

Product Science Overview

Antibodies

Antibodies, also known as immunoglobulins (Ig), are large, Y-shaped proteins used by the immune system to identify and neutralize antigens such as bacteria and viruses . Each antibody binds to a specific antigen in a highly specific interaction analogous to a lock and key .

Structure and Function

Antibodies are composed of two identical light (L) chains and two identical heavy (H) chains, which are held together by disulfide bonds to form a flexible Y shape . Each chain is composed of a variable (V) region and a constant © region . The tips of the Y-shaped structure contain paratopes that specifically bind to epitopes on antigens, allowing the two molecules to bind together with precision .

Classes and Functions

Antibodies occur in five classes, sometimes called isotypes: IgA, IgD, IgE, IgG, and IgM . Each class has different functions and is released in different parts of the body at various stages of an immune response . For example, IgG is the most common antibody in blood and extracellular fluid, providing the majority of antibody-based immunity against invading pathogens .

Medical Applications

Antibodies are crucial for the immune response and are used in various medical applications, including diagnostics and therapeutics . Monoclonal antibodies, which are identical antibodies produced by a single clone of cells, are used to treat diseases such as cancer and autoimmune disorders .

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