MAT2A Antibody
Methionine Adenosyltransferase II Alpha, Mouse Anti Human
Cat. No.
BT21010
Source
Synonyms
MATA2, MATII, SAMS2, MAT-2A, S-adenosylmethionine synthetase isoform type-2, AdoMet synthetase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT2A, AMS2.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
MAT2A is a crucial enzyme in cellular metabolism responsible for catalyzing the synthesis of S-adenosylmethionine (SAMe) from L-methionine and ATP. Primarily found in extrahepatic tissues, MAT2A expression in the liver is linked to growth, dedifferentiation, and cancer development. Transcription factors NF-kappa B and AP-1 are essential for both basal MAT2A expression in HepG2 cells and its upregulation in response to TNF-alpha. Increased MAT2A expression has been shown to enhance growth, while s-adenosylmethionine and methylthioadenosine can inhibit mitogenic signaling in colon cancer cells. Conversely, reduced expression of both MAT2A and MAT2beta can disrupt leptin signaling pathways in liver cancer cells.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be stored at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
The MAT2A antibody has been validated for use in ELISA, Western blot, and immunohistochemistry applications, demonstrating its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application and should be determined empirically. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000. For immunohistochemistry, a dilution range of 1:50 to 1:100 is recommended, with a starting dilution of 1:50.
Synonyms
MATA2, MATII, SAMS2, MAT-2A, S-adenosylmethionine synthetase isoform type-2, AdoMet synthetase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT2A, AMS2.
Purification Method
MAT2A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT3A2AT.
Immunogen
Anti-human MAT2A mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAT2A amino acids 1-395 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Function and Importance
Expression and Regulation
Research and Applications
MAT2A has been studied extensively for its role in various biological processes and its potential implications in diseases. For instance, it has been identified as a pivotal factor for zygotic genome activation (ZGA) in mouse embryos . Additionally, MAT2A is involved in the response to low-dose gamma irradiation, leading to increased expression of the long noncoding RNA PARTICLE .
Mouse Anti Human MAT2A Antibody
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