MAPK8 Human

Mitogen-Activated Protein Kinase 8 Human Recombinant

Cat. No.

BT12146

Source

E.coli.

Synonyms

Mitogen-activated protein kinase 8, MAPK8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK1, PRKM8, SAPK1, SAPK1C, Mitogen-activated protein kinase 8 JNK beta 2, JNK, JNK1A2, JNK21B1/2.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MAPK8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 450 amino acids (1-427a.a) and having a molecular mass of 50.5kDa.
MAPK8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Mitogen-Activated Protein Kinase 8, also known as MAPK8, is a member of the MAP kinase family. MAP kinases serve as a central point for integrating various biochemical signals involved in a wide range of cellular processes. The MAPK8 gene encodes a protein that plays a role in UV radiation-induced apoptosis, which is linked to the cytochrome c-mediated cell death pathway.

Description

Recombinant Human MAPK8, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 450 amino acids (1-427a.a) with a molecular weight of 50.5 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear and colorless solution that has been sterilized by filtration.

Formulation

The MAPK8 solution is provided at a concentration of 0.5 mg/ml and contains Phosphate Buffered Saline (pH 7.4), 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (2-4 weeks), keep the solution at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.

Purity

The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.

Synonyms

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSRSKRD NNFYSVEIGD STFTVLKRYQ NLKPIGSGAQ GIVCAAYDAI LERNVAIKKL SRPFQNQTHA KRAYRELVLM KCVNHKNIIG LLNVFTPQKS LEEFQDVYIV MELMDANLCQ VIQMELDHER MSYLLYQMLC GIKHLHSAGI IHRDLKPSNI VVKSDCTLKI LDFGLARTAG TSFMMTPYVV TRYYRAPEVI LGMGYKENVD IWSVGCIMGE MIKGGVLFPG TDHIDQWNKV IEQLGTPCPE FMKKLQPTVR TYVENRPKYA GYSFEKLFPD VLFPADSEHN KLKASQARDL LSKMLVIDAS KRISVDEALQ HPYINVWYDP SEAEAPPPKI PDKQLDEREH TIEEWKELIY KEVMDLEERT KNGVIRGQPS PLGAAVINGS QHPSSSSSVN DVSSMSTDPT LASDTDSSLE AAAGPLGCCR.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase 8 (MAPK8), also known as c-Jun N-terminal kinase 1 (JNK1), is a crucial member of the MAP kinase family. This family of proteins plays a significant role in various cellular processes, including proliferation, differentiation, transcription regulation, and development. MAPK8 is particularly notable for its involvement in stress responses and apoptosis.

Structure and Isoforms

MAPK8 is a serine/threonine protein kinase that localizes to the cytoplasm. It can activate both the MAP kinase and JNK kinase pathways. The gene encoding MAPK8 can produce multiple isoforms through alternative splicing, which allows for diverse functional roles within the cell. These isoforms include JNK1 Alpha1 and JNK1 Beta2, among others.

Activation and Function

MAPK8 is activated by various cell stimuli, including cytokines, growth factors, and environmental stresses such as UV radiation. Upon activation, MAPK8 targets specific transcription factors, leading to the immediate-early gene expression in response to these stimuli. This kinase is essential for mediating cellular responses to stress and is involved in processes such as apoptosis and inflammation.

One of the critical pathways involving MAPK8 is the tumor necrosis factor-alpha (TNF-alpha) induced apoptosis pathway. MAPK8 activation is required for TNF-alpha-induced apoptosis, highlighting its role in programmed cell death. Additionally, MAPK8 is involved in UV radiation-induced apoptosis, which is thought to be related to the cytochrome c-mediated cell death pathway.

Clinical Significance

MAPK8 has been implicated in various diseases, including cancer and inflammatory conditions. Its role in apoptosis and stress responses makes it a potential target for therapeutic interventions. For instance, inhibiting MAPK8 activity could be beneficial in conditions where excessive apoptosis is detrimental, such as neurodegenerative diseases.

Recombinant MAPK8

Recombinant MAPK8 is produced using recombinant DNA technology, which involves inserting the MAPK8 gene into an expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in research to study the kinase’s function, its role in various signaling pathways, and its potential as a therapeutic target.

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MAPK8 Human

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