MAP2K6 Human

Mitogen-Activated Protein Kinase Kinase 6 Human Recombinant
Cat. No.
BT11252
Source
Escherichia Coli.
Synonyms
Mitogen-Activated Protein Kinase Kinase 6, PRKMK6, MEK6, MKK6, Stress-Activated Protein Kinase Kinase 3,MAPK/ERK Kinase 6, SAPK Kinase 3, EC 2.7.12.2, MAPKK, SAPKK, SAPKK3, MEK 6, Protein Kinase, Mitogen-Activated, Kinase 6 (MAP Kinase Kinase 6), Dual Specificity Mitogen-Activated Protein Kinase Kinase 6, Kinase 6 (MAP Kinase Kinase 6), MAP Kinase Kinase 6,Mitogen-Activated, Protein Kinase, SKK3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAP2K6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (53-314 a.a) and having a molecular mass of 32kDa.MAP2K6 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mitogen-Activated Protein Kinase 6 (MAP2K6) is a member of the dual specificity protein kinase family. It plays a crucial role in activating p38 MAP kinase in response to stress signals and inflammatory cytokines. As a key component of the MAP kinase signaling pathway, MAP2K6 is involved in various cellular processes, including stress-induced apoptosis and cell cycle arrest.
Description
Recombinant human MAP2K6, expressed in E. coli, is a purified, non-glycosylated polypeptide chain. It consists of 283 amino acids (residues 53-314) with a molecular weight of 32 kDa. The protein includes a 21 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The MAP2K6 protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Mitogen-Activated Protein Kinase Kinase 6, PRKMK6, MEK6, MKK6, Stress-Activated Protein Kinase Kinase 3,MAPK/ERK Kinase 6, SAPK Kinase 3, EC 2.7.12.2, MAPKK, SAPKK, SAPKK3, MEK 6, Protein Kinase, Mitogen-Activated, Kinase 6 (MAP Kinase Kinase 6), Dual Specificity Mitogen-Activated Protein Kinase Kinase 6, Kinase 6 (MAP Kinase Kinase 6), MAP Kinase Kinase 6,Mitogen-Activated, Protein Kinase, SKK3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLEPIMELGR GAYGVVEKMR HVPSGQIMAV KRIRATVNSQ EQKRLLMDLD ISMRTVDCPF TVTFYGALFR EGDVWICMEL MDTSLDKFYK QVIDKGQTIP EDILGKIAVS IVKALEHLHS KLSVIHRDVK PSNVLINALG QVKMCDFGIS GYLVDEVAKE IDAGCKPYMA PERINPELNQ KGYSVKSDIW SLGITMIELA ILRFPYDSWG TPFQQLKQVV EEPSPQLPAD KFSAEFVDFT SQCLKKNSKE RPTYPELMQH PFF.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6), also known as MKK6, is a crucial component of the MAP kinase signal transduction pathway. This pathway is essential for various cellular processes, including cell growth, differentiation, and response to environmental stress. MAP2K6 is a dual specificity protein kinase, meaning it can phosphorylate both threonine and tyrosine residues on its substrate proteins .

Gene and Protein Structure

The MAP2K6 gene is located on chromosome 17 in humans. It encodes a protein that is approximately 334 amino acids in length. The protein structure includes several key domains that are essential for its kinase activity, including an ATP-binding site and a serine/threonine kinase domain .

Function

MAP2K6 plays a pivotal role in the activation of p38 MAP kinases (MAPK11, MAPK12, MAPK13, and MAPK14). These kinases are involved in the cellular response to inflammatory cytokines and environmental stress. Upon activation, MAP2K6 phosphorylates p38 MAP kinases, which then go on to activate various transcription factors involved in stress response, cell cycle arrest, and apoptosis .

Biological Pathways

MAP2K6 is involved in several critical biological pathways:

  • Stress Response: It is activated in response to environmental stressors such as UV radiation, heat shock, and osmotic stress.
  • Inflammatory Response: MAP2K6 is activated by pro-inflammatory cytokines like TNF-α and IL-1β, leading to the activation of p38 MAP kinases and subsequent inflammatory responses.
  • Cell Cycle Regulation: It plays a role in cell cycle arrest, particularly in response to DNA damage or other cellular stressors .
Clinical Significance

Mutations or dysregulation of MAP2K6 have been associated with various diseases, including cancer and inflammatory disorders. For instance, alterations in the MAP2K6 gene have been linked to colorectal cancer and congenital myopathy . Understanding the role of MAP2K6 in these diseases can provide insights into potential therapeutic targets.

Recombinant MAP2K6

Human recombinant MAP2K6 is produced using recombinant DNA technology, which involves inserting the human MAP2K6 gene into a suitable expression system, such as bacteria or yeast. This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant MAP2K6 is used in various biochemical assays to study its function and interactions with other proteins .

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