MAP2K1 Human
MAP2K1, MEK1, PRKMK1, MKK1, MAPKK 1, MAP kinase kinase 1.
Greater than 90.0% as determined by SDS-PAGE.
Description
MAP2K1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 402 amino acids (1-393a.a.) and having a molecular mass of 44.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).MAP2K1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
MAP2K1 protein is encoded by the MAP2K1 gene. This enzyme, part of the dual specificity protein kinase family, functions as a mitogen-activated protein (MAP) kinase. Extracellular signal-regulated kinases, including MAP kinases, play a crucial role in integrating various biochemical signals. Acting upstream of MAP kinases, MAP2K1 activates them in response to multiple intra- and extracellular signals. As a key component of the MAP kinase signal transduction pathway, this enzyme participates in cellular processes such as transcription regulation, proliferation, and differentiation.
Produced in Sf9 Baculovirus cells, MAP2K1 is a single, glycosylated polypeptide chain composed of 402 amino acids (1-393a.a.) with a molecular mass of 44.5 kDa. Note that on SDS-PAGE, its molecular size appears between 40-57 kDa. This MAP2K1 protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The MAP2K1 protein solution is provided at a concentration of 0.25 mg/ml and contains 10% glycerol in Phosphate Buffered Saline (pH 7.4).
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
MAP2K1, MEK1, PRKMK1, MKK1, MAPKK 1, MAP kinase kinase 1.
ADPMPKKKPT PIQLNPAPDG SAVNGTSSAE TNLEALQKKL EELELDEQQR KRLEAFLTQK QKVGELKDDD FEKISELGAG NGGVVFKVSH KPSGLVMARK LIHLEIKPAI RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKK AGRIPEQILG KVSIAVIKGL TYLREKHKIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR SYMSPERLQG THYSVQSDIW SMGLSLVEMA VGRYPIPPPD AKELELMFGC QVEGDAAETP PRPRTPGRPL SSYGMDSRPP MAIFELLDYI VNEPPPKLPS GVFSLEFQDF VNKCLIKNPA ERADLKQLMV HAFIKRSDAE EVDFAGWLCS TIGLNQPSTP THAAGVHHHH HH.
Product Science Overview
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), also known as MEK1, is a crucial component of the MAPK/ERK pathway, which is involved in various cellular processes such as proliferation, differentiation, and survival. This pathway is highly conserved across species and plays a significant role in transmitting extracellular signals to the nucleus, thereby influencing gene expression and cellular responses.
MAP2K1 is a dual-specificity kinase, meaning it can phosphorylate both serine/threonine and tyrosine residues. It specifically phosphorylates and activates the MAP kinases ERK1 and ERK2. The activation of MAP2K1 itself is regulated by upstream kinases, such as RAF, which phosphorylate MAP2K1 on specific serine residues. Once activated, MAP2K1 phosphorylates ERK1/2, leading to their activation and subsequent translocation to the nucleus, where they phosphorylate various transcription factors and other target proteins .
The MAPK/ERK pathway, including MAP2K1, is involved in numerous cellular processes:
- Cell Proliferation: It promotes cell division and growth by regulating the expression of genes involved in the cell cycle.
- Differentiation: It influences the differentiation of various cell types, including neurons and muscle cells.
- Survival: It plays a role in cell survival by inhibiting apoptotic pathways.
- Development: It is essential for normal embryonic development and tissue homeostasis.
Dysregulation of MAP2K1 and the MAPK/ERK pathway is associated with various diseases, including cancer. Mutations in MAP2K1 can lead to its constitutive activation, resulting in uncontrolled cell proliferation and tumorigenesis. Inhibitors targeting MAP2K1, such as trametinib, are used in the treatment of certain cancers, including melanoma .
Human recombinant MAP2K1 is produced using recombinant DNA technology, which involves inserting the human MAP2K1 gene into an expression vector and introducing it into a host cell, such as E. coli or yeast. The host cells then produce the MAP2K1 protein, which can be purified and used for various research and therapeutic purposes. Recombinant MAP2K1 is used in studies to understand its function, regulation, and role in disease, as well as in the development of MAP2K1 inhibitors for therapeutic use.
