MAGED1 Human

Melanoma Antigen Family D, 1 Human Recombinant
Cat. No.
BT6628
Source
E.coli.
Synonyms
MAGED1, Melanoma Antigen Family D 1, Neurotrophin Receptor-Interacting, MAGE Homolog, NRAGE, MAGE Tumor Antigen CCF, MAGE-D1 Antigen, DLXIN-1, Melanoma-Associated Antigen D1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAGED1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (504-760 a.a) and having a molecular mass of 31.7kDa.
MAGED1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Melanoma-associated antigen D1 (MAGED1), a member of the melanoma antigen gene (MAGE) family, exhibits expression in nearly all normal adult tissues. This protein plays a role in the p75 neurotrophin receptor-mediated programmed cell death pathway. MAGED1 is implicated in Prader-Willi syndrome, a genetic disorder characterized by hyperphagia, repetitive and compulsive behaviors, and cognitive impairment. It participates in the apoptotic response triggered by nerve growth factor (NGF) binding in neuronal cells. MAGED1 influences cell cycle progression and facilitates NGFR-mediated apoptosis. Furthermore, it acts as a regulator of DLX family member function and contributes to circadian rhythm regulation.
Description
Recombinant human MAGED1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 280 amino acids (residues 504-760). With a molecular weight of 31.7 kDa, this protein comprises amino acids 504 to 760 of the MAGED1 sequence. A 23-amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The MAGED1 protein is provided in a solution with a concentration of 0.25 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, and 1 mM DTT.
Stability
For optimal storage, the product should be kept at 4°C if it will be used within 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein, such as 0.1% HSA or BSA, is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the MAGED1 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
MAGED1, Melanoma Antigen Family D 1, Neurotrophin Receptor-Interacting, MAGE Homolog, NRAGE, MAGE Tumor Antigen CCF, MAGE-D1 Antigen, DLXIN-1, Melanoma-Associated Antigen D1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP.

Product Science Overview

Introduction

Melanoma Antigen Family D, 1 (MAGE-D1) is a member of the melanoma-associated antigen (MAGE) family, which is known for its role in various cellular processes, including cell cycle regulation, apoptosis, and tumor progression. The MAGE family is divided into two subfamilies: type I and type II. MAGE-D1 belongs to the type II subfamily, which is expressed in a wide range of tissues and is not restricted to tumors.

Structure and Function

MAGE-D1 is a protein encoded by the MAGE-D1 gene located on chromosome Xq28. The protein consists of 778 amino acids and has a molecular weight of approximately 87 kDa. MAGE-D1 contains several functional domains, including a MAGE homology domain (MHD) and a proline-rich region, which are crucial for its interactions with other proteins.

MAGE-D1 is involved in various cellular processes, such as:

  • Cell cycle regulation: MAGE-D1 interacts with cell cycle regulators, including p53 and p21, to control cell proliferation.
  • Apoptosis: MAGE-D1 can promote apoptosis by interacting with pro-apoptotic proteins, such as Bax and Bak.
  • Tumor progression: MAGE-D1 is implicated in the progression of various cancers, including melanoma, by modulating cell migration, invasion, and metastasis.
Expression and Regulation

MAGE-D1 is ubiquitously expressed in normal tissues, including the brain, heart, liver, and kidney. Its expression is regulated by various factors, such as transcription factors, epigenetic modifications, and post-translational modifications. In cancer cells, MAGE-D1 expression is often dysregulated, leading to altered cellular functions and tumor progression.

Clinical Significance

MAGE-D1 has been studied extensively for its potential as a therapeutic target and biomarker in cancer. Its expression is associated with poor prognosis in several cancers, including melanoma, breast cancer, and lung cancer. Targeting MAGE-D1 with small molecules, antibodies, or RNA-based therapies has shown promise in preclinical studies, highlighting its potential for cancer treatment.

Human Recombinant MAGE-D1

Recombinant MAGE-D1 is produced using recombinant DNA technology, which involves the insertion of the MAGE-D1 gene into an expression vector, followed by the expression of the protein in a suitable host system, such as E. coli or mammalian cells. The recombinant protein is then purified using various chromatographic techniques to obtain a highly pure and biologically active product.

Recombinant MAGE-D1 is used in various research applications, including:

  • Functional studies: Investigating the role of MAGE-D1 in cellular processes, such as cell cycle regulation, apoptosis, and tumor progression.
  • Drug discovery: Screening for small molecules or antibodies that target MAGE-D1 for potential therapeutic applications.
  • Biomarker development: Developing assays to detect MAGE-D1 expression in clinical samples for diagnostic and prognostic purposes.

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