MAGEA5 Human

Melanoma Antigen Family A, 5 Human Recombinant
Cat. No.
BT6319
Source
E.coli.
Synonyms
Melanoma antigen family A 5, Cancer/testis antigen 1.5, CT1.5, cancer/testis antigen family 1 member 5, MAGE-5 (5a/5b) antigen, MAGEA4, MGC129526.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAGEA5 Human Recombinant produced in E. coli is a single polypeptide chain containing 148 amino acids (1-124) and having a molecular mass of 15.6kDa.
MAGEA5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The MAGEA5 gene belongs to the MAGEA gene family, which consists of genes encoding proteins with a high degree of sequence similarity (50-80%). The variability observed in the promoters and first exons of MAGEA genes suggests that this gene family allows for diverse transcriptional control mechanisms while potentially maintaining a conserved function. Notably, MAGEA genes cluster at the Xq28 chromosomal location and have been linked to several hereditary disorders, including dyskeratosis congenita. MAGEA5 encodes a protein with a truncated C-terminus compared to other family members, leading to interpretations suggesting it might be a pseudogene.
Description
Recombinant MAGEA5, produced in E. coli, is a single polypeptide chain comprising 148 amino acids (residues 1-124) with a molecular weight of 15.6 kDa. The protein includes a 24 amino acid His-tag fused at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
The MAGEA5 solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 mM PMSF, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of MAGEA5 is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
Melanoma antigen family A 5, Cancer/testis antigen 1.5, CT1.5, cancer/testis antigen family 1 member 5, MAGE-5 (5a/5b) antigen, MAGEA4, MGC129526.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSLEQK SQHCKPEEGL DTQEEALGLV GVQAATTEEQ EAVSSSSPLV PGTLGEVPAA GSPGPLKSPQ GASAIPTAID FTLWRQSIKG SSNQEEEGPS TSPDPESVFR AALSKKVADL IHFLLLKY

Product Science Overview

Introduction

Melanoma Antigen Family A (MAGE-A) proteins are a subgroup of the cancer/testis (CT) antigens, which are typically expressed in various cancers and immune-privileged tissues such as the testis. Among these, MAGE-A5 is a notable member due to its potential role in cancer immunotherapy.

Discovery and Structure

The MAGE-A family was first identified in the early 1990s when researchers discovered MAGE-A1, the first cancer/testis antigen, through the recognition of a melanoma cell line by autologous cytotoxic CD8 T cells . MAGE-A proteins share a common MAGE homology domain, which is crucial for their function and interaction with other cellular proteins .

Expression and Function

MAGE-A5, like other MAGE-A proteins, is predominantly expressed in germline cells and a variety of cancers, including melanoma, bladder cancer, and others . This restricted expression pattern makes MAGE-A5 an attractive target for cancer immunotherapy. The protein is involved in several cellular processes, including cell cycle regulation, apoptosis, and DNA repair .

Role in Cancer

MAGE-A5 is associated with increased cancer cell proliferation, survival, and resistance to various therapies . Its expression in tumors is often correlated with poor prognosis and aggressive disease. This makes MAGE-A5 a valuable biomarker for cancer diagnosis and a potential target for therapeutic interventions .

Therapeutic Potential

Given its restricted expression in normal tissues and high expression in tumors, MAGE-A5 is a promising target for cancer immunotherapy. Strategies such as cancer vaccines, adoptive T-cell transfer, and combination therapies are being explored to target MAGE-A5 and other MAGE-A proteins . These approaches aim to enhance the immune system’s ability to recognize and destroy cancer cells expressing MAGE-A5.

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