MAGEA4 Human

Melanoma Antigen Family A, 4 Human Recombinant
Cat. No.
BT6215
Source
Escherichia Coli.
Synonyms
CT1.4, MAGE4, MAGE-4 antigen, MAGE-X2 antigen, MAGE-41 antigen, MAGE4B, MGC21336, Cancer/testis antigen 1.4, MAGEA4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAGEA4 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37 kDa. The MAGEA4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Belonging to the MAGEA family, MAGEA4 plays a role in the expression of tumor antigens recognized by autologous cytolytic T lymphocytes (CTL) on human melanoma cells. This gene is involved in embryonic development and processes related to tumor transformation and progression. MAGEA4 expression is observed in various tumor types, including melanoma, head and neck squamous cell carcinoma, lung carcinoma, and breast carcinoma. However, it is not typically expressed in normal tissues, except for the testes and placenta.
Description
Recombinant Human MAGEA4, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 337 amino acids (1-317 a.a.). With a molecular weight of 37 kDa, this protein includes a 20 amino acid His-Tag fused at the N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MAGEA4 Human solution is supplied in a buffer containing 20mM Tris-HCl at pH 8 and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity greater than 90.0%.
Synonyms
CT1.4, MAGE4, MAGE-4 antigen, MAGE-X2 antigen, MAGE-41 antigen, MAGE4B, MGC21336, Cancer/testis antigen 1.4, MAGEA4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPTSNTYTLV TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI AYPSLREAAL LEEEEGV.

Product Science Overview

Introduction

Melanoma Antigen Family A, 4 (MAGE-A4) is a member of the melanoma-associated antigen (MAGE) family, which is a subclass of cancer/testis (CT) antigens. These antigens are characterized by their restricted expression in immune-privileged tissues such as the testis and placenta, and their aberrant expression in various cancers .

Discovery and Structure

The MAGE family was first identified in the early 1990s, with MAGE-A4 being one of the 12 family A MAGE genes identified by De Plaen et al. in 1994 . The MAGE-A4 gene encodes a 317-amino acid polypeptide . The protein structure includes a conserved MAGE homology domain, which is shared among the MAGE family members .

Expression and Function

MAGE-A4 is expressed in a variety of cancer cell lines, including melanoma, sarcoma, and bladder cancer, but is typically not found in normal tissues except for the testis and placenta . This restricted expression pattern makes MAGE-A4 an attractive target for cancer immunotherapy .

The primary function of MAGE-A4 in normal tissues is not well understood. However, in cancer cells, MAGE-A4 is associated with increased cell proliferation, survival, and resistance to various therapies . This makes it a valuable biomarker and therapeutic target in cancer treatment .

Therapeutic Implications

Given its restricted expression in normal tissues and widespread expression in various cancers, MAGE-A4 has been explored as a target for cancer immunotherapy. Strategies include cancer vaccines, adoptive T-cell transfer, and combination therapies . Clinical trials targeting MAGE-A4 have shown promising results, suggesting that it could play a significant role in the future of cancer treatment .

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THE BIOTEK
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