Lymphotactin Rat
Escherichia Coli.
XCL1, Cytokine SCM-1, ATAC, Lymphotaxin, SCM-1-alpha, Small inducible cytokine C1, XC chemokine ligand 1, LTN, LPTN, SCM1, SCM-1, SCYC1, SCM-1a.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Lymphotactin (XCL1) Rat Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 93 amino acids and having a molecular mass of approximately 10.0kDa.
Lymphotactin is purified by proprietary chromatographic techniques.
Product Specs
Lymphotactin, also known as XCL1, is a small cytokine classified under the XC chemokine family. This chemokine is primarily found in the spleen, thymus, intestine, and peripheral blood leukocytes, with lower concentrations observed in the lungs, prostate gland, and ovary. Lymphotactin is mainly produced by activated CD8+ T cells in the thymus and peripheral blood. It plays a crucial role in attracting T cells. Notably, XCL1 exhibits a close relationship with XCL2 in humans, with both genes situated at the same locus on chromosome 1. The chemotactic function of XCL1 is mediated through its binding to a chemokine receptor known as XCR1.
Recombinant Rat Lymphotactin (XCL1) is a non-glycosylated polypeptide chain produced in E. coli. It comprises 93 amino acids and has a molecular weight of approximately 10.0 kDa. The purification process of Lymphotactin involves proprietary chromatographic methods.
The product appears as a sterile, white lyophilized (freeze-dried) powder.
The product is lyophilized from a 0.2 µm filtered solution concentrated in phosphate-buffered saline (PBS) at pH 7.4.
To reconstitute the lyophilized Lymphotactin, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Lyophilized XCL1 can be stored at room temperature for up to 3 weeks, but for long-term storage, it is best to keep it desiccated at a temperature below -18°C. Once reconstituted, Lymphotactin should be stored at 4°C for a period of 2-7 days. For future use, store it below -18°C. Avoid repeated freeze-thaw cycles.
The purity of the product is greater than 97.0%, as determined by the following methods:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
The half-maximal effective concentration (ED50) of the product is determined using a chemotaxis bioassay with human XCR1 transfected murine BaF3 cells. The ED50 is less than 100 ng/ml, which corresponds to a specific activity greater than 1.0 × 104 IU/mg.
XCL1, Cytokine SCM-1, ATAC, Lymphotaxin, SCM-1-alpha, Small inducible cytokine C1, XC chemokine ligand 1, LTN, LPTN, SCM1, SCM-1, SCYC1, SCM-1a.
Escherichia Coli.
VGTEVLQESI CVSLRTQRLP VQKIKTYTIK EGAMRAVIFV TKRGLRICAD PQAKWVKTAI KTVDGRASAS KSKAETIPTQ AQRSASTAVT LTG.
Product Science Overview
Lymphotactin is known for its chemotactic activity, specifically attracting lymphocytes and NK cells . It exerts its function by binding to the chemokine receptor XCR1, which is uniquely expressed on cross-presenting dendritic cells . This interaction is critical for the immune response, as it helps in the targeting and activation of these immune cells.
Studies have shown that the N-terminal region of XCL1 stabilizes its binding to the XCR1 receptor, which is essential for its chemotactic function . Interestingly, the N-terminus does not contain critical elements for inducing chemotaxis but rather limits the chemotactic action at higher concentrations . The C-terminus, on the other hand, does not play a significant role in receptor binding or chemotaxis .
