Lymphotactin Human
Lymphotactin Human Recombinant (XCL1)
Cat. No.
BT16575
Source
Escherichia Coli.
Synonyms
XCL1, Cytokine SCM-1, ATAC, Lymphotaxin, SCM-1-alpha, Small inducible cytokine C1, XC chemokine ligand 1, LTN, LPTN, SCM1, SCM-1, SCYC1, SCM-1a.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Lymphotactin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 92 amino acids and having a molecular mass of 10007 Dalton.
The Lymphotactin is purified by proprietary chromatographic techniques.
The Lymphotactin is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Lymphotactin, also known as chemokine (C motif) ligand (XCL1), is a small cytokine classified under the XC chemokine family. This cytokine is primarily found in the spleen, thymus, intestine, and peripheral blood leukocytes, with lower concentrations detected in the lung, prostate gland, and ovary. Activated thymic and peripheral blood CD8+ T cells are identified as cellular sources for XCL1. The primary function of this chemokine involves attracting T cells. In humans, XCL1 exhibits a close relationship with another chemokine, XCL2, both of which are encoded by genes located at the same locus on chromosome 1. XCL1 exerts its chemotactic influence by binding to a chemokine receptor known as XCR1.
Description
Recombinant Human Lymphotactin, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 92 amino acids. With a molecular weight of 10.007 kDa, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The XCL1 protein was lyophilized from a concentrated solution (1 mg/ml) in water, free from any additives.
Solubility
To reconstitute the lyophilized Lymphotactin, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted into other aqueous solutions as required.
Stability
Lyophilized Lymphotactin, though stable at room temperature for 3 weeks, should be stored desiccated below -18°C. After reconstitution, XCL1 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store below -18°C after adding a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.
Purity
The purity is determined to be greater than 99.0% using the following methods: (a) RP-HPLC analysis (b) SDS-PAGE analysis
Biological Activity
The biological activity of Lymphotactin is determined by its chemoattractant properties towards human T cells. At concentrations of 10-100 ng/ml, it demonstrates a specific activity of 10,000-100,000 IU/mg.
Synonyms
XCL1, Cytokine SCM-1, ATAC, Lymphotaxin, SCM-1-alpha, Small inducible cytokine C1, XC chemokine ligand 1, LTN, LPTN, SCM1, SCM-1, SCYC1, SCM-1a.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Glu-Val-Ser.
Product Science Overview
Gene and Protein Structure
Biological Activity
Applications in Research and Medicine
Recombinant human lymphotactin is widely used in research to study immune responses and inflammation. It is also used in various assays to understand its role in chemotaxis and its potential therapeutic applications. For instance, it can be used to investigate the mechanisms of immune cell migration and to develop treatments for conditions involving immune cell dysfunction .
Production and Storage
Recombinant human lymphotactin is typically produced in a carrier-free form, meaning it does not contain bovine serum albumin (BSA). This is important for applications where the presence of BSA could interfere with experimental results . The protein is lyophilized from a filtered solution and can be reconstituted in sterile PBS for use. It is stable for up to 12 months when stored at -20 to -70°C and for up to 3 months after reconstitution .
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