LY9 Human
Lymphocyte Antigen 9 Human Recombinant
Cat. No.
BT3954
Source
Sf9, Baculovirus cells.
Synonyms
Lymphocyte Antigen 9, Signaling Lymphocytic Activation Molecule 3, Cell Surface Molecule Ly-9, SLAM Family Member 3, SLAMF3, CD229 Antigen, CD229, Hly9, MLY9, LY9.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LY9 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 416 amino acids (48-454 aa) and having a molecular mass of 45.9kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).
LY9 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
LY9 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Lymphocyte Antigen 9, also known as LY9, belongs to the SLAM family of immunomodulatory receptors. LY9 interacts with the SAP adaptor molecule and plays a role in adhesion reactions between T lymphocytes and accessory cells through homophilic interaction. It promotes the differentiation of T-cells into a Th17 helper T-cell phenotype, leading to increased IL-17 secretion; this costimulatory activity requires SH2D1A. Additionally, LY9 promotes the recruitment of RORC to the IL-17 promoter. It is involved in maintaining peripheral cell tolerance by acting as a negative regulator of the immune response. LY9 suppresses autoantibody responses and inhibits IFN-gamma secretion by CD4(+) T-cells. Furthermore, it negatively regulates the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells. Systemic Lupus Erythematosus is among the diseases associated with LY9.
Description
Recombinant human LY9 protein, produced in Sf9 Baculovirus cells, is a single glycosylated polypeptide chain containing 416 amino acids (48-454 aa). It has a molecular mass of 45.9 kDa and migrates at 40-57 kDa on SDS-PAGE under reducing conditions. LY9 is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
LY9 protein solution at a concentration of 0.25 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the entire vial at 4°C. For longer storage, freeze at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0% as determined by SDS-PAGE.
Synonyms
Lymphocyte Antigen 9, Signaling Lymphocytic Activation Molecule 3, Cell Surface Molecule Ly-9, SLAM Family Member 3, SLAMF3, CD229 Antigen, CD229, Hly9, MLY9, LY9.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADLKDSAPTV VSGILGGSVT LPLNISVDTE IENVIWIGPK NALAFARPKE NVTIMVKSYL GRLDITKWSY SLCISNLTLN DAGSYKAQIN QRNFEVTTEE EFTLFVYEQL QEPQVTMKSV KVSENFSCNI TLMCSVKGAE KSVLYSWTPR EPHASESNGG SILTVSRTPC DPDLPYICTA QNPVSQRSSL PVHVGQFCTD PGASRGGTTG ETVVGVLGEP VTLPLALPAC RDTEKVVWLF NTSIISKERE EAATADPLIK SRDPYKNRVW VSSQDCSLKI SQLKIEDAGP YHAYVCSEAS SVTSMTHVTL LIYRRLRKPK ITWSLRHSED GICRISLTCS VEDGGNTVMY TWTPLQKEAV VSQGESHLNV SWRSSENHPN LTCTASNPVS RSSHQFLSEN ICSGPERNTK HHHHHH
Product Science Overview
Structure and Expression
LY9 is a glycosylated polypeptide chain consisting of 416 amino acids . It is expressed on the surface of various immune cells, including T lymphocytes, B lymphocytes, and natural killer (NK) cells . The protein is involved in homophilic interactions, meaning it can bind to other LY9 molecules on adjacent cells .
Function
LY9 has several important functions in the immune system:
- T-Cell Differentiation: LY9 promotes the differentiation of T-cells into a helper T-cell Th17 phenotype, leading to increased secretion of interleukin-17 (IL-17) .
- Immune Regulation: It acts as a negative regulator of the immune response, helping to maintain peripheral cell tolerance and prevent autoimmunity .
- Adhesion: LY9 participates in adhesion reactions between T lymphocytes and accessory cells, facilitating cell-cell interactions .
- Inhibition of IFN-gamma Secretion: It inhibits the secretion of interferon-gamma (IFN-γ) by CD4+ T-cells, which is crucial for controlling inflammatory responses .
Clinical Significance
Recombinant LY9
Recombinant LY9 is produced using Sf9 Baculovirus cells and is available for research purposes . It is typically supplied as a sterile, filtered, colorless solution containing phosphate-buffered saline (PBS) and glycerol . The recombinant protein is used in various laboratory assays to study its function and role in immune regulation.
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