LSM2 Antibody

LSM2 Homolog, U6 Small Nuclear RNA Associated, Mouse Anti Human
Cat. No.
BT23896
Source
Synonyms
LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.
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Description

Product Specs

Introduction
LSM2, part of the snRNP Sm protein family, belongs to a group of proteins identified in various organisms based on their similarity to the Sm protein family. These Sm-like proteins share a conserved Sm sequence motif, consisting of two regions separated by a variable-length linker that folds into a loop structure. It is believed that Sm-like proteins assemble into a stable heteromer found in tri-snRNP particles, which play a crucial role in pre-mRNA splicing. LSM2 specifically binds to the 3'-terminal U-tract of U6 snRNA and participates in the pre-mRNA splicing process.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be stored at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Repeated freezing and thawing should be avoided.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
The LSM2 antibody has been validated for use in ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined through titration for each specific application. For Western blot analysis, a dilution range of 1:1,000 to 1:3,000 is recommended.
Synonyms
LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.
Purification Method
LSM2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2B2AT.
Immunogen
Anti-human LSM2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human LSM2 amino acids 1-95 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Introduction

LSM2 (LSM2 Homolog, U6 Small Nuclear RNA Associated) is a protein-coding gene that plays a crucial role in RNA processing and degradation. This gene is part of the LSm (Like Sm) family of RNA-binding proteins, which are essential for various cellular processes, including pre-mRNA splicing and mRNA decay.

Gene and Protein Structure

The LSM2 gene is located on chromosome 6 in humans and encodes a protein that is a component of the U6 small nuclear ribonucleoprotein (snRNP) complex . The protein is highly conserved across different species, including mice, where it is also known as Lsm2 . The LSM2 protein is characterized by its ability to bind to the 3’-terminal oligo(U) tract of U6 snRNA, which is crucial for its function in RNA processing .

Function

LSM2 is involved in several key cellular processes:

  1. Pre-mRNA Splicing: LSM2 is a component of the U4/U6-U5 tri-snRNP complex, which is essential for the assembly of the spliceosome . The spliceosome is responsible for the removal of introns from pre-mRNA, a critical step in the maturation of mRNA.
  2. mRNA Degradation: LSM2 also plays a role in the degradation of mRNA by forming stable heteromers with other LSm proteins . This process is vital for the regulation of gene expression and the maintenance of cellular homeostasis.
  3. RNA Binding: The LSM2 protein binds specifically to the 3’-terminal U-tract of U6 snRNA, facilitating the formation of the U4/U6 snRNP complex .
Biological Pathways

LSM2 is involved in several biological pathways, including:

  • Processing of Capped Intron-Containing Pre-mRNA: This pathway involves the modification and splicing of pre-mRNA to form mature mRNA .
  • Deadenylation-Dependent mRNA Decay: This pathway is responsible for the degradation of mRNA molecules that have been marked for decay, ensuring the proper regulation of gene expression .
Clinical Significance

Mutations or dysregulation of the LSM2 gene have been associated with various diseases, including mixed connective tissue disease and cat-scratch disease . Understanding the function and regulation of LSM2 is crucial for developing potential therapeutic strategies for these conditions.

Research and Applications

Research on LSM2 has provided valuable insights into its role in RNA processing and its potential implications in disease. The use of mouse models has been instrumental in studying the function of LSM2 and its homologs in different species . Additionally, antibodies targeting LSM2, such as mouse anti-human LSM2 antibodies, are widely used in research to study the protein’s expression and function in various cellular contexts .

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