LIPG Human, HEK
Lipase Endothelial Human Recombinant, HEK
Cat. No.
BT5423
Source
HEK 293.
Synonyms
LIPG, Lipase Endothelial, EDL, EL, Endothelial Cell-Derived Lipase, EC 3.1.1.3, PRO719, Endothelial Lipase, Lipoprotein Lipase H, EC 3.1.1.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LIPG Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ser21-Pro500) containing a total of 490 amino acids, having a calculated molecular mass of 55.8kDa. LIPG is fused to a 2 aa N-terminal linker, a 2 aa C-terminal linker and a 6 aa His tag at C-Terminus.
Product Specs
Introduction
Lipase Endothelial (LIPG) is an enzyme with significant phospholipase activity that may play a role in lipoprotein metabolism and vascular health. It is classified as a member of the TG lipase family due to its sequence and a unique 'lid region,' which determines substrate specificity for enzymes within this family. LIPG also exhibits triglyceride lipase activities and demonstrates a higher efficiency in hydrolyzing HDLs compared to other lipoproteins. Additionally, LIPG has the ability to bind heparin.
Description
Recombinant human LIPG, produced in HEK cells, is a single, glycosylated polypeptide chain consisting of amino acids Ser21 to Pro500 (total 490 amino acids). It has a calculated molecular mass of 55.8 kDa. This LIPG protein includes a 2 amino acid N-terminal linker, a 2 amino acid C-terminal linker, and a 6 amino acid His tag located at the C-terminus.
Physical Appearance
White powder, lyophilized (freeze-dried), and filtered.
Formulation
The LIPG protein was subjected to filtration (using a 0.4 µm filter) and lyophilized from a solution containing phosphate buffered saline at pH 7.5 (PBS), 1% (w/v) Sucrose, and 4% (w/v) Mannitol.
Solubility
To prepare a working stock solution, it is advised to add deionized water to the lyophilized pellet aiming for a concentration of approximately 0.5 mg/ml. Allow the pellet to dissolve completely. Important: This LIPG product is not sterile. Prior to use in cell culture, it is essential to filter the solution through a sterile filter of appropriate pore size.
Stability
The lyophilized protein should be stored at -20°C. After reconstitution, it is recommended to aliquot the product to avoid repeated cycles of freezing and thawing. The reconstituted protein can be stored at 4°C for a limited time; stability studies indicate no change in protein quality after two weeks at 4°C.
Purity
Purity determined by SDS-PAGE is greater than 85.0%.
Synonyms
LIPG, Lipase Endothelial, EDL, EL, Endothelial Cell-Derived Lipase, EC 3.1.1.3, PRO719, Endothelial Lipase, Lipoprotein Lipase H, EC 3.1.1.
Source
HEK 293.
Amino Acid Sequence
ASSPVPFGPE GRLEDKLHKP KATQTEVKPS VRFNLRTSKD PEHEGCYLSV GHSQPLEDCS FNMTAKTFFI IHGWTMSGIF ENWLHKLVSA LHTREKDANV VVVDWLPLAH QLYTDAVNNT RVVGHSIARM LDWLQEKDDF SLGNVHLIGY SLGAHVAGYA GNFVKGTVGR ITGLDPAGPM FEGADIHKRL SPDDADFVDV LHTYTRSFGL SIGIQMPVGH IDIYPNGGDF QPGCGLNDVL GSIAYGTITE VVKCEHERAV HLFVDSLVNQ DKPSFAFQCT DSNRFKKGIC LSCRKNRCNS IGYNAKKMRN KRNSKMYLKT RAGMPFRVYH YQMKIHVFSY KNMGEIEPTF YVTLYGTNAD SQTLPLEIVE RIEQNATNTF LVYTEEDLGD LLKIQLTWEG ASQSWYNLWK EFRSYLSQPR NPGRELNIRR IRVKSGETQR KLTFCTEDPE NTSISPGREL WFRKCRDGWR MKNETSPTVE LP KLHHHHHH.
Product Science Overview
Structure and Production
Lipase Endothelial (LIPG) is produced as a recombinant protein in HEK 293 cells. The recombinant form is a single, glycosylated polypeptide chain consisting of 490 amino acids, with a calculated molecular mass of approximately 55.8 kDa . The protein is fused to a 6 amino acid His tag at the C-terminus, which facilitates its purification .
Function and Activity
LIPG exhibits extensive phospholipase activity and is more active as a phospholipase than a triglyceride lipase . It hydrolyzes high-density lipoproteins (HDL) more efficiently than other lipoproteins, which is significant for its role in lipoprotein metabolism . Additionally, LIPG binds heparin, which may influence its activity and interactions within the vascular system .
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