LHRH Protein

Luteinizing Hormone Releasing Hormone Human Recombinant
Cat. No.
BT15172
Source
Escherichia Coli.
Synonyms

LHRH, GRH, GNRH, LNRH.

Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LHRH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 92 amino acids (24-92 a.a.) and having a molecular mass of 10.3kDa.
LHRH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Gonadotropin-releasing hormone 1 (GNRH1), also known as Luteinizing-hormone releasing hormone (LHRH), is a crucial peptide hormone that regulates reproductive function. Produced and released by the hypothalamus, GNRH1 acts on the anterior pituitary gland to stimulate the synthesis and secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency of GNRH1 pulses determines the balance between FSH and LH release, with low frequency favoring FSH and high frequency favoring LH. This pulsatile secretion pattern is essential for proper reproductive function in both males and females. In females, GNRH1 secretion varies across the menstrual cycle, with a surge occurring just before ovulation. This surge is critical for follicular growth, ovulation, and corpus luteum maintenance. In males, consistent GNRH1 pulses support spermatogenesis.
Description
This product consists of recombinant human LHRH protein produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 92 amino acids (specifically, amino acids 24-92). The protein has a molecular mass of 10.3 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of the LHRH protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The LHRH protein solution is provided at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and also contains 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For optimal product stability: If using the entire vial within 2-4 weeks, store at 4°C. For longer-term storage, freeze at -20°C. To further enhance long-term stability, adding a carrier protein such as 0.1% HSA or BSA is recommended. Repeated freezing and thawing of the product should be avoided.
Purity
SDS-PAGE analysis indicates that the purity of this product is greater than 90%.
Synonyms

LHRH, GRH, GNRH, LNRH.

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQHWSYGL RPGGKRDAEN LIDSFQEIVK EVGQLAETQR FECTTHQPRS PLRDLKGALE SLIEEETGQK KI.

Product Science Overview

Introduction

Luteinizing Hormone Releasing Hormone (LHRH), also known as Gonadotropin-Releasing Hormone (GnRH), is a pivotal hormone in the regulation of the reproductive system. It is a decapeptide produced in the hypothalamus and plays a crucial role in the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the anterior pituitary gland .

Structure and Function

LHRH is a small peptide hormone consisting of ten amino acids. Its primary function is to stimulate the synthesis and release of LH and FSH, which are essential for reproductive function in both males and females. In males, LH stimulates the production of testosterone from the Leydig cells in the testes, while in females, it triggers ovulation and the production of estrogen and progesterone from the ovaries .

Recombinant LHRH

Recombinant LHRH (Human Recombinant) is a synthetic form of the naturally occurring hormone. It is produced using recombinant DNA technology, which involves inserting the gene encoding LHRH into bacterial or mammalian cells, allowing these cells to produce the hormone. This method ensures a consistent and pure product, which is crucial for therapeutic applications .

Clinical Applications

Recombinant LHRH is used in various clinical settings, particularly in the treatment of hormone-responsive cancers such as prostate and breast cancer. It is also used in assisted reproductive technology (ART) to regulate ovulation and improve the outcomes of in vitro fertilization (IVF) procedures .

  1. Cancer Treatment: In hormone-responsive cancers, recombinant LHRH is used to suppress the production of sex hormones that can fuel the growth of cancer cells. By continuously stimulating the pituitary gland, recombinant LHRH eventually leads to a decrease in LH and FSH levels, resulting in reduced production of testosterone and estrogen .

  2. Assisted Reproductive Technology: In ART, recombinant LHRH is used to control the timing of ovulation and improve the chances of successful fertilization. It is often used in combination with other hormones such as recombinant human follicle-stimulating hormone (r-hFSH) to enhance ovarian stimulation and increase the number of oocytes retrieved .

Advantages of Recombinant LHRH

The use of recombinant LHRH offers several advantages over naturally derived hormones:

  • Purity and Consistency: Recombinant technology ensures a high level of purity and consistency, reducing the risk of contamination and variability.
  • Safety: Recombinant LHRH is free from pathogens that could be present in hormones extracted from human or animal sources.
  • Efficacy: The recombinant form is highly effective in achieving the desired therapeutic outcomes, whether in cancer treatment or assisted reproduction .

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