LHPP Human
Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase Human Recombinant
Cat. No.
BT29327
Source
Escherichia Coli.
Synonyms
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, LHPP, HDHD2B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LHPP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (1-270) and having a molecular mass of 33.5kDa.
LHPP is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LHPP is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) is a member of the HAD-like hydrolase superfamily. This enzyme is unique because it can hydrolyze both oxygen-phosphorus bonds, such as those found in inorganic pyrophosphate, and nitrogen-phosphorus bonds, like those in phospholysine, phosphohistidine, and imidodiphosphate. LHPP is found in several tissues, with high concentrations in the liver and kidney, and moderate levels in the brain.
Description
Recombinant human LHPP, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 307 amino acids, with amino acids 1-270 representing the LHPP protein, and has a molecular weight of 33.5 kDa. A 37 amino acid His-tag is fused to the N-terminus of the protein to facilitate purification, which is carried out using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile solution.
Formulation
The LHPP solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the LHPP solution can be stored at 4°C. For extended storage, it is recommended to store the solution at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Repeated freezing and thawing of the solution should be minimized.
Purity
The purity of LHPP is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, LHPP, HDHD2B.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHP WYASMTGGQQ MGRDLYDDDD KDRWGSHMAP WGKRLAGVRG VLLDISGVLY DSGAGGGTAI AGSVEAVARL KRSRLKVRFC TNESQKSRAE LVGQLQRLGF DISEQEVTAP APAACQILKE QGLRPYLLIH DGVRSEFDQI DTSNPNCVVI ADAGESFSYQ NMNNAFQVLM ELEKPVLISL GKGRYYKETS GLMLDVGPYM KALEYACGIK AEVVGKPSPE FFKSALQAIG VEAHQAVMIG DDIVGDVGGA QRCGMRALQV RTGKFRPSDE HHPEVKADGY VDNLAEAVDL LLQHADK.
Product Science Overview
Gene and Protein Structure
Biological Function
LHPP plays a crucial role in various biological processes, including:
- Phosphate Metabolism: It is involved in the hydrolysis of phosphate-containing compounds .
- Tumor Suppression: Recent studies have identified LHPP as a novel tumor suppressor. It inhibits cell proliferation, growth, migration, invasion, and metabolism in various cancers . Specifically, in gastric cancer, LHPP suppresses the expression of the insulin-like growth factor 1 receptor (IGF1R), thereby inhibiting cell adhesion and proliferation .
Clinical Significance
Research and Applications
Human recombinant LHPP is used in various research applications to study its function and role in disease. It is particularly significant in cancer research due to its tumor-suppressing properties . Researchers utilize various assays, including RNA sequencing and Gene Set Enrichment Analysis, to explore the mechanisms through which LHPP regulates cell behavior .
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