LGALS9 Human

Galectin-9 Human Recombinant
Cat. No.
BT11597
Source
Escherichia Coli.
Synonyms
Lectin galactoside-binding soluble 9, Urate transporter/channel protein, LGALS9A, MGC125973, HUAT, Ecalectin, Galectin-9, MGC117375, MGC125974, HOM-HD-21, LGALS9.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids (1-148 a.a.) and having a molecular mass of 18.5 kDa.
Galectin-9 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
LGALS9, also known as Galectin-9, exhibits a strong binding affinity for galactosides, particularly the Forssman pentasaccharide. This lectin plays a crucial role in thymocyte-epithelial cell interactions within the thymus, influencing its biological processes. LGALS9 demonstrates an inhibitory effect on cell proliferation and serves as a ligand for HAVCR2/TIM3. Furthermore, it induces apoptosis in T helper type 1 lymphocytes (Th1) and acts as an eosinophil chemoattractant. As an S-type lectin, LGALS9 is overexpressed in Hodgkin's disease tissue, contributing to interactions between Hodgkin and Reed-Sternberg (H&RS) cells and their surrounding cellular environment.
Description
Recombinant LGALS9, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 168 amino acids (1-148 a.a.). With a molecular weight of 18.5 kDa, this protein is fused to a 20-amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The LGALS9 protein solution is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 100mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of LGALS9 is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Lectin galactoside-binding soluble 9, Urate transporter/channel protein, LGALS9A, MGC125973, HUAT, Ecalectin, Galectin-9, MGC117375, MGC125974, HOM-HD-21, LGALS9.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAFSGSQAPY LSPAVPFSGT IQGGLQDGLQ ITVNGTVLSS SGTRFAVNFQ TGFSGNDIAF HFNPRFEDGG YVVCNTRQNG SWGPEERKTH MPFQKGMPFD LCFLVQSSDF KVMVNGILFV QYFHRVPFHR VDTISVNGSV QLSYISFQ.

Product Science Overview

Discovery and Structure

Galectin-9 was first isolated from mouse embryonic kidney in 1997 as a 36 kDa beta-galactoside lectin protein . It contains two carbohydrate recognition domains (CRDs) connected by a linker peptide, making it a tandem-repeat type galectin . The human recombinant form of Galectin-9 is typically produced in E. coli and has a molecular weight of approximately 34-37 kDa .

Biological Functions

Galectin-9 plays a significant role in various biological processes:

  • Immune Regulation: It is known to induce apoptosis in T-helper type 1 lymphocytes (Th1) by binding to its receptor, HAVCR2/TIM3 . This interaction is crucial for immune tolerance and the suppression of autoimmune responses.
  • Cell Adhesion and Migration: Galectin-9 binds to galactosides and has a high affinity for the Forssman pentasaccharide . It interferes with the associations of CD44, VCAM-1, and Integrin α4β1, thereby suppressing tumor cell metastasis .
  • Thymocyte-Epithelial Interactions: It plays a role in thymocyte-epithelial interactions, which are essential for the development and function of the thymus .
Clinical and Research Applications

Recombinant human Galectin-9 is widely used in research to study its effects on cell proliferation, apoptosis, and immune responses. It has been shown to support the adhesion of Jurkat cells, a type of human T cell leukemia . Additionally, it is used to investigate its potential therapeutic applications in cancer and autoimmune diseases.

Production and Storage

Recombinant human Galectin-9 is typically produced in E. coli and purified to a high degree of purity (>95%) using SDS-PAGE under reducing conditions . The protein is lyophilized and can be stored at -20°C to -80°C for up to 12 months. Once reconstituted, it should be stored at 4-8°C for short-term use or at -20°C for longer-term storage .

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LGALS9 Mouse
Galectin-9 Mouse Recombinant
Cat. No.
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Source
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THE BIOTEK
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