LGALS7 Mouse

Galectin-7 Mouse Recombinant
Cat. No.
BT11299
Source
Escherichia Coli.
Synonyms
Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein, LGALS7, PIG1, LGALS7B, GAL7, LGALS7A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS7 mouse Recombinant produced E. coli is a single polypeptide chain containing 159 amino acids (1-136) and having a molecular mass of 17.6kDa.
LGALS7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Galectins are a family of carbohydrate-binding proteins found in animals that exhibit a specific affinity for beta-galactosides. This family consists of at least 14 known members, all sharing similarities in their carbohydrate recognition domain (CRD), which is responsible for binding to carbohydrates. Galectins are initially produced within the cell's cytoplasm and lack a traditional signal peptide for secretion. However, they can be secreted through non-classical pathways or directed to the nucleus. These proteins play a role in regulating interactions between cells and between cells and the extracellular matrix. Human Galectin-7, specifically, is a prototypical galectin with a single CRD and was first discovered in human skin cells. Its expression is triggered by the tumor suppressor protein p53 and is linked to programmed cell death (apoptosis). Galectin-7 promotes apoptosis by acting within the cell, upstream of JNK activation and mitochondrial cytochrome c release. Its association with UV-induced apoptosis in skin cells suggests a crucial role in maintaining healthy skin. In human cells, Galectin-7 can be found in both the nucleus and the cytoplasm.
Description
Recombinant LGALS7 from mouse, produced in E. coli, is a single polypeptide chain comprising 159 amino acids (1-136) with a molecular weight of 17.6kDa. This protein includes a 23 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The LGALS7 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 20% glycerol, and 2mM DTT.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein, LGALS7, PIG1, LGALS7B, GAL7, LGALS7A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSATQHK TSLPQGVRVG TVMRIRGMVP DQAGRFHVNL LCGEEQGADA ALHFNPRLDT SEVVFNTKEQ GKWGREERGT GIPFERGQPF EVLLIATEEG FKAVVGDDEY LHFHHRMPPA RVRLVEVGGD VQLHSVKIF.

Product Science Overview

Introduction

Galectin-7 is a member of the galectin family, which consists of proteins that bind to β-galactoside sugars. These proteins play crucial roles in various cellular processes, including cell-cell adhesion, cell-matrix interactions, and apoptosis. Galectin-7, in particular, is known for its involvement in the regulation of apoptosis and its expression in epithelial tissues.

Structure and Expression

Galectin-7 is a 14 kDa protein encoded by the LGALS7 gene. The recombinant form of mouse Galectin-7 is typically expressed in Escherichia coli and purified to a high degree of purity, often exceeding 95% as determined by SDS-PAGE . The protein consists of 136 amino acids and includes a His tag at the N-terminus to facilitate purification .

Biological Functions

Galectin-7 is primarily known for its pro-apoptotic functions. It acts upstream of the JNK activation and mitochondrial cytochrome c release, which are critical steps in the apoptosis pathway . This protein is particularly important in the context of UV-induced apoptosis of keratinocytes, playing a vital role in maintaining epidermal homeostasis .

Applications

Recombinant mouse Galectin-7 is widely used in research to study its role in apoptosis and other cellular processes. It is suitable for various applications, including SDS-PAGE and mass spectrometry (MS) . Researchers often use this protein to investigate the mechanisms underlying cell death and survival, as well as its potential implications in diseases such as cancer.

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THE BIOTEK
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