LGALS4 Mouse

Galectin-4 Mouse Recombinant
Cat. No.
BT11025
Source
Escherichia Coli.
Synonyms
gal-4 , Galectin-4, Lactose-binding lectin 4, lectin galactoside-binding soluble 4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (1-326a.a) and having a molecular mass of 38.8kDa.LGALS4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Galectin-4, a member of the galectin family, consists of two carbohydrate recognition domains with similar peptide chains. Galectins are proteins that bind to beta-galactosides and play a role in regulating cell-cell and cell-matrix interactions. This function allows them to inhibit chronic inflammation, graft-versus-host disease (GVHD), and allergic responses. LGALS4, specifically, is mainly found in the small intestine, colon, and rectum, with reduced expression observed in colorectal cancer. It binds to glycosphingolipids containing 3-O-sulfated Gal residues and cholesterol-3-sulfate as endogenous ligands. Involved in cell adhesion, LGALS4 crosslinks the lateral cell membranes of surface-lining epithelial cells in the intestines, maintaining epithelial integrity against mechanical stress. Additionally, it regulates intestinal inflammation by selectively controlling the cell cycle and apoptosis of peripheral and mucosal T cells, contributing to T-cell death through a caspase-independent pathway. Notably, blocking LGALS4 can reduce T-cell death induced by TNF-alpha inhibitors. LGALS4 also suppresses the secretion of pro-inflammatory cytokines, such as IL-6 and IL-17.
Description
Recombinant LGALS4 from mouse, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 349 amino acids (1-326a.a) with a molecular weight of 38.8 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The LGALS4 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the entire vial can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. To preserve protein integrity, minimize repeated freeze-thaw cycles.
Purity
The purity of the LGALS4 protein is greater than 90%, as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of LGALS4 is assessed based on its ability to agglutinate human red blood cells. The ED50, which represents the effective concentration for inducing 50% agglutination, is determined to be less than 5 ug/ml.
Synonyms
gal-4 , Galectin-4, Lactose-binding lectin 4, lectin galactoside-binding soluble 4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAYVPAP GYQPTYNPTL PYKRPIPGGL SVGMSVYIQG MAKENMRRFH VNFAVGQDDG ADVAFHFNPR FDGWDKVVFN TMQSGQWGKE EKKKSMPFQK GKHFELVFMV MPEHYKVVVN GNSFYEYGHR LPVQMVTHLQ VDGDLELQSI NFLGGQPAAA PYPGAMTIPA YPAGSPGYNP PQMNTLPVMT GPPVFNPRVP YVGALQGGLT VRRTIIIKGY VLPTARNFVI NFKVGSSGDI ALHLNPRIGD SVVRNSFMNG SWGAEERKVA YNPFGPGQFF DLSIRCGMDR FKVFANGQHL FDFSHRFQAF QMVDTLEING DITLSYVQI.

Product Science Overview

Introduction

Galectin-4 is a member of the galectin family, which consists of carbohydrate-binding proteins with a specific affinity for N-acetyl-lactosamine-containing glycoproteins . These proteins play crucial roles in various biological processes, including cell-cell adhesion, cell-matrix interactions, and intracellular signaling.

Structure and Function

Galectin-4 is a 36 kDa protein characterized by its tandem-repeat structure, meaning it contains two carbohydrate recognition domains (CRDs) within a single polypeptide chain . Each CRD binds to different carbohydrate groups, including those found on erythrocyte blood group antigens . This dual CRD structure allows Galectin-4 to cross-link glycoproteins and glycolipids on the cell surface, facilitating cell adhesion and signaling.

Expression and Localization

Galectin-4 is predominantly expressed in the gastrointestinal tract, where it plays a role in maintaining the integrity of the epithelial barrier . It is also found in well-differentiated breast and liver carcinomas, suggesting a potential role in cancer biology . The presence of Galectin-4 in these tissues indicates its involvement in both normal physiological processes and pathological conditions.

Biological Activities

One of the key activities of Galectin-4 is its ability to agglutinate human red blood cells, a property that has been utilized in various experimental assays . This agglutination activity is dose-dependent, with an effective dose (ED50) ranging from 1.25 to 16.0 µg/mL . Additionally, Galectin-4 has been implicated in modulating immune responses, influencing cell migration, and promoting cell proliferation.

Recombinant Production

Recombinant Mouse Galectin-4 is typically produced using Escherichia coli (E. coli) expression systems . The recombinant protein is purified to a high degree of purity (>95%) and is free from endotoxins (<0.01 EU per 1 µg of protein) . The recombinant form retains the biological activities of the native protein, making it a valuable tool for research and therapeutic applications.

Applications in Research

Recombinant Mouse Galectin-4 is widely used in research to study its role in various biological processes and diseases. It is employed in cell culture experiments to investigate cell adhesion, migration, and signaling pathways. Additionally, it serves as a standard in enzyme-linked immunosorbent assays (ELISAs) and other biochemical assays to quantify Galectin-4 levels in biological samples.

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LGALS4 Human
Galectin-4 Human Recombinant
Cat. No.
BT10946
Source
Escherichia Coli.
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