Introduction
Galectin-4, a member of the galectin family, consists of two carbohydrate recognition domains with similar peptide chains. This family of beta-galactoside-binding proteins plays a crucial role in regulating cell-cell and cell-matrix interactions, ultimately inhibiting chronic inflammation, graft-versus-host disease (GVHD), and allergic responses. LGALS4 expression is primarily observed in the small intestine, colon, and rectum, with underexpression noted in colorectal cancer. Functionally, LGALS4 acts as an endogenous ligand for glycosphingolipids containing 3-O-sulfated Gal residues and binds to cholesterol-3-sulfate, contributing to cell adhesion. It plays a vital role in crosslinking the lateral cell membranes of surface-lining epithelial cells, enhancing epithelial integrity against mechanical stress within the bowel. Furthermore, LGALS4 influences intestinal inflammation by selectively regulating the cell cycle and apoptosis of peripheral and mucosal T-cells through a caspase-independent pathway. Notably, LGALS4 blockade has been shown to reduce TNF-alpha inhibitor-induced T-cell death, and it can decrease the secretion of pro-inflammatory cytokines like IL-6 and IL-17.
Description
Produced in E. coli, this Galectin-4 is a non-glycosylated polypeptide chain comprising 343 amino acids (with amino acids 1-323 present) and possessing a molecular weight of 38.1 kDa. A 20 amino acid His-Tag is fused to the N-terminus of the protein, which is then purified using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
This LGALS4 protein solution is formulated with 20mM Tris-HCl at a pH of 8, 1mM DTT, and 10% glycerol.
Stability
For optimal storage, keep at 4°C if the entire vial will be utilized within 2-4 weeks. For longer storage, freeze at -20°C. To further ensure stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is recommended. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of this product is greater than 95%, as assessed by SDS-PAGE.
Synonyms
Galectin-4, Gal-4, Lactose-binding lectin 4, L-36 lactose-binding protein, L36LBP, Antigen NY-CO-27, LGALS4, lectin galactoside-binding soluble 4, GAL4.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR LSAFQRVDTL EIQGDVTLSY VQI.
